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OspC Antibody

Rabbit Polyclonal
NCI Collaboration
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Western blot showing detection of 0.1 µg of recombinant OspC protein.  Lane 1: Molecular weight markers.  Lane 2:  MBP-OspC fusion protein (arrow; expected MW: 63.1 kDa).  Lane 3:  MBP alone.  Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose.  After blocking with 1% BSA-TTBS (p/n  MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min.  HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Number:
200-401-C11
Size:
100 µg
Price:
$309.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

OspC Antibody Properties

Description
Anti-OspC (RABBIT) Antibody - 200-401-C11
Host
Rabbit
Target Species
Borrelia burgdorferi
Known Cross Reactivity
Borrelia burgdorferi
Clonality
Polyclonal
Application
Western Blot : 1:1,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
ospC, BB_B19
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OspC Antibody Description

Background
Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane.
Synonyms
OspC, Borrelia burgdorferi OspC, PC, Outer Surface Protein C
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi OspC protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein-A purified antibody has been tested for use in Western blotting and in ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspC protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi, afzelii, and valaisiana sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. japonica and americana sources based on 94% homology. Cross-reactivity with OspC from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Jauris-Heipke S., Liegl G., Preac-Mursic V., Roessler D., Schwab E., Soutschek E., Will G., Wilske B. (1995) Molecular analysis of genes encoding outer surface protein C (OspC) of Borrelia burgdorferi sensu lato: relationship to ospA genotype and evidence of lateral gene exchange of ospC. J. Clin. Microbiol. 33:1860-1866 [PubMed: 7665660] [Abstract] Livey I., Gibbs C.P., Schuster R., Dorner F. (1995) Evidence for lateral transfer and recombination in OspC variation in Lyme disease Borrelia. Mol. Microbiol. 18:257-269 [PubMed: 8709845] [Abstract]
Specific Reference
Casselli, T., Tourand, Y., & Bankhead, T. (2012). Altered murine tissue colonization by Borrelia burgdorferi following targeted deletion of linear plasmid 17-carried genes. Infection and immunity, 80(5), 1773-1782.
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-310

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-310

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
111-1102

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
111-3102

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry