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Primary Antibodies  >  Infectious Disease Antibodies
 

OspA Antibody

Rabbit Polyclonal
Code

200-401-C13
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot showing detection of 0.1 µg of recombinant OspA protein.  Lane 1: Molecular weight markers.  Lane 2:  MBP-OspA fusion protein (arrow; expected MW: 70.5 kDa).  Lane 3:  MBP alone.  Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose.  After blocking with 1% BSA-TTBS (p/n  MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min.  HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Synonyms: Outer surface protein A, Borrelia burgdorferi OspA

OspA Antibody Properties

Description
Anti-OspA (RABBIT) Antibody - 200-401-C13
Host
Rabbit
Target Species
Borrelia burgdorferi
Known Cross Reactivity
Borrelia burgdorferi
Clonality
Polyclonal
Application
ELISA : >1:5,000
Western Blot : 1:1,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
ospA, BB_A15
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Lot#  

OspA Antibody Description

Background
Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi encoded on its Plasmid lp54, is a major component of the spirochete's extracellular matrix. OspA probably serves as a lipid-anchor. The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression.
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi OspA protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 28.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspA protein. A BLAST analysis was used to suggest cross-reactivity with OspA from B. burgdorferi and sources based on 100% homology with the immunizing sequence. Cross-reactivity with OspA or Osp from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Norris SJ. The dynamic proteome of Lyme disease Borrelia. (2006) Genome Biol. 7(3):209. Epub 2006 Mar 17. Ding W., Huang X., Yang X., Dunn J.J., Luft B.J., Koide S., Lawson C.L. (2000) Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA. J. Mol. Biol. 302:1153-1164(2000) [PubMed: 11183781] [Abstract] Stevenson B, Tilly K, and Rosa P.A. (1996) A Family of Genes Located on Four Separate 32-Kilobase Circular Plasmids in Borrelia burgdorferi B31. J Bacteriol 178(12) June 1996 p3508-3516
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-310

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-310

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
111-1102

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
111-3102

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry