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Primary Antibodies  >  Infectious Disease Antibodies
 

OspB Antibody

Rabbit Polyclonal
Code

200-401-C15
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot showing detection of 0.1 µg of recombinant OspB protein.  Lane 1: Molecular weight markers.  Lane 2:  MBP-OspB fusion protein (arrow; expected MW = 72.7 kDa).  Lane 3:  MBP alone.  Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose.  After blocking with 1% BSA-TTBS (p/n  MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min.  HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Synonyms: Outer surface protein B, Borrelia burgdorferi OspB

OspB Antibody Properties

Description
Anti-OspB (RABBIT) Antibody - 200-401-C15
Host
Rabbit
Target Species
Borrelia burgdorferi
Known Cross Reactivity
Borrelia burgdorferi
Clonality
Polyclonal
Application
ELISA : 1:13,000
Western Blot : 1:1,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
ospB, locus BB_A16
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Lot#  

OspB Antibody Description

Background
OspB, is one of the major Outer Surface Proteins of the outer membrane of Borrelia burgdorferi, which is composed of various unique outer surface proteins (Osp) that have been characterized (OspA through OspF). The Osp proteins are lipoproteins anchored by N-terminally attached fatty acid molecules to the membrane. They are presumed to play a role in virulence, transmission, or survival in the tick. Two of the major surface Ag of Borrelia burgdorferi, the 31-kDa OspA and 34-kDa OspB proteins, show a high degree of sequence similarity, are encoded by a 49-kb plasmid and share a common promoter, and are coordinately transcribed. OspA, OspB, and OspD are expressed by B. burgdorferi residing in the gut of unfed ticks, suggesting that they promote the persistence of the spirochete in ticks between blood meals. OspB has a contributing role in the adherence of B. burgdorferi to the tick gut. The C terminus of OspB is important for eliciting a protective immune response to OspB. B. burgdorferi has the ability to vary its surface proteins in response to immune attack.
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi OspB protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 30.3 kDa in size corresponding to Borrelia burgdorferi OspB protein by Western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspB protein. A BLAST analysis was used to suggest cross-reactivity with OspB from B. burgdorferi, afzelii, spielmanii, and garinii sources based on 100% homology with the immunizing sequence, and with B. valaisiana based on 99% homology. Cross-reactivity with OspB from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Haake DA (2000). Spirochaetal lipoproteins and pathogenesis. Microbiology 146 (Pt 7): 1491-1504. Neelakanta G, Li X, Pal U, Liu X, Beck DS, DePonte K, Fish D, Kantor FS, and Fikrig E (2007). Outer surface protein B is critical for Borrelia burgdorferi adherence and survival within Ixodes ticks. PLoS Pathog. 3 (3): e33. doi:10.1371/journal.ppat.0030033. Liang FT, Caimano MJ, Radolf JD, Fikrig E. (2004) Borrelia burgdorferi outer surface protein (osp) B expression independent of ospA. Microb Pathog. Jul;37(1):35-40.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-310

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-310

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
111-1102

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
111-3102

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry