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Primary Antibodies  >  Infectious Disease Antibodies
 

CRASP-1 Antibody

Rabbit Polyclonal
Code

200-401-C18
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot showing detection of 0.1 µg of recombinant CRASP-1 protein.  Lane 1: Molecular weight markers.  Lane 2:  MBP-CRASP-1 fusion protein (arrow; expected MW = 69.3 kDa).  Lane 3:  MBP alone.  Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose.  After blocking with 1% BSA-TTBS (p/n  MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min.  HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Synonyms: Complement regulator acquiring protein 1, Borrelia burgdorferi CRASP-1

CRASP-1 Antibody Properties

Description
Anti-CRASP-1 (RABBIT) Antibody - 200-401-C18
Host
Rabbit
Target Species
Borrelia burgdorferi
Known Cross Reactivity
Borrelia burgdorferi
Clonality
Polyclonal
Application
ELISA : >1:5,000
Western Blot : 1:1,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CRASP1
Download CofA
Lot#  

CRASP-1 Antibody Description

Background
CRASP-1, or Complement Regulator-Acquiring Surface Protein 1, is a multifunctional protein of Lyme disease-causing B. burgdorferi that binds to several human extracellular matrix proteins and plasminogen, including factor H (resulting in inhibition of complement activation in mammals) and Human Bone Morphogenic Protein 2. These interactions may contribute to adhesion, bacterial colonization, and organ tropism and may allow dissemination of B. burgdorferi in the host. B. burgdorferi spirochetes express up to 5 complement regulator-acquiring surface proteins. Multiple copies of sequences analagous to CRASP-1 genes have been detected in Borrelia plasmids. Borrelia species contain a large number of plasmids, of linear and circular, some of which appear to repeat sequences or contain fragments of other genes. These regions may serve as potentially usable information for the survival of Borrelia in its multiple environments during its life cycle. In addition, the sequence for CRASP-1 contains a repeated sequence folded into a stable stem loop structure typical of RNA genes.
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi CRASP-1 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 26.9 kDa in size corresponding to Borrelia burgdorferi CRASP-1 protein by Western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi CRASP-1 protein. A BLAST analysis was used to suggest reactivity with CRASP-1 from B. burgdorferi and B. garinii sources based on 100% homology with the immunizing sequence. Partial cross-reactivity is expected against B. spielmanii, afzelii, and valaisiana sources based on 98% homology. Cross-reactivity with CRASP-1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Delihas N. (2009) Intergenic regions of Borrelia plasmids contain phylogenetically conserved RNA secondary structure motifs. BMC Genomics. 2009 Mar 6;10:101. Hallström T, Haupt K, Kraiczy P, Hortschansky P, Wallich R, Skerka C, Zipfel PF. (2010) Complement regulator-acquiring surface protein 1 of Borrelia burgdorferi binds to human bone morphogenic protein 2, several extracellular matrix proteins, and plasminogen. J Infect Dis. 2010 Aug 15;202(3):490-8. Kraiczy P, Hellwage J, Skerka C, Becker H, Kirschfink M, Simon MM, Brade V, Zipfel PF, and Wallich R (2004) Complement Resistance of Borrelia burgdorferi Correlates with the Expression of BbCRASP-1, a Novel Linear Plasmid-encoded Surface Protein That Interacts with Human Factor H and FHL-1 and Is Unrelated to Erp Proteins. J Biol Chem, 279, 2421-2429.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-301

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-319

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
100-4164P

Product Type
Primary Antibodies;
Applications
ELISA, WB
Catalog Number
K-711

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry