House Secondary Banner
 
You may also want to consider these related products.
IL-1ß Human Recombinant Protein - 009-001-301
IL-1ß Human Recombinant Protein - 009-001-301
IL-1ß Mouse Recombinant Protein - 010-001-319
IL-1ß Mouse Recombinant Protein - 010-001-319
Anti-RABBIT IgG (H&L) (GOAT) Antibody - 111-1102
Anti-RABBIT IgG (H&L) (GOAT) Antibody - 111-1102
Anti-RABBIT (H&L) (MOUSE) Antibody - 111-3102
Anti-RABBIT (H&L) (MOUSE) Antibody - 111-3102
Primary Antibodies  >  Infectious Disease Antibodies
 

VlsE Antibody

Rabbit Polyclonal
Code

200-401-C33
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western blot showing detection of 0.1 µg of recombinant VlsE protein.  Lane 1: Molecular weight markers.  Lane 2:  MBP-VlsE fusion protein (arrow; expected MW: 78.8 kDa).  Lane 3:  MBP alone.  Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose.  After blocking with 1% BSA-TTBS (p/n  MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min.  HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad).
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Outer surface protein VlsE, Borrelia burgdorferi VlsE, vlsE protein

VlsE Antibody Properties

Description
Anti-VlsE (Rabbit) Antibody - 200-401-C33
Host
Rabbit
Target Species
Borrelia burgdorferi
Known Cross Reactivity
Borrelia burgdorferi
Clonality
Polyclonal
Application
ELISA : 1:250
Western Blot : 1:1000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Specific Activity
0
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
vlsE
Download CofA
Lot#  

VlsE Antibody Description

Background
Variable Lipoprotein Surface-Exposed protein, or VlsE, is a lipoprotein on the surface of the Lyme Disease spirochete Borrelia burgdorferi, detectable during all its life stages. It can exist as many different isoforms. VlsE has variable regions (VRs) and invariable regions (IRs). Some IRs are anchored in the outer membrane of the bacteria and some are antigens exposed on the membrane surface. Replacement of the VR by Borrelia within days of being transferred to a mammalian host presents new surface antigens to the host immune system, and helps Borrelia avoid a strong reaction by host immune systems. The VlsE is apparently not modified as much in the tick or in the rodent vector, when compared to in the mammal host. Several putative envelope proteins of B. burgdorferi appear to be expressed only in the infected mammalian host. The VRs are antigenic, irregularly shaped loops on the bacterial surface which may help to hide both membrane-incorporated and surface portions of adjacent proteins from immune cells. These VR loops are coded by antigenic cassettes. The protein loops can therefore be switched in or out of the protein, or different type loops traded. In B. burgdorferi there seem to be at least fifteen different VlsE cassettes that can insert into any of the variable regions of VlsE, allowing it to appear as millions of different antigens. Similar, but smaller, systems also operate for OSP-A, OSP-B, OSP-C, and other proteins. Some current research involves determination of control of cassette activation. One IR region, C6, of the VlsE protein, consistently stimulates a strong immune response. Its presentation may be a decoy that misdirects the immune system from less protected sites by causing competion for binding antibodies. The bound antibodies are thus not available for binding important therapeutic proteins. This may help Borrelia to enter T-cells, leading to their destruction. Because IR6 is invariable and found in all life stages of B. burgdorferi, it has been used in an ELISA diagnostic test for early IgM of Lyme Disease.
Immunogen
MBP recombinant protein corresponding to Borrelia burgdorferi VlsE protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-VlsE antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~36.3 kDa in size corresponding to VlsE by Western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi VlsE protein. A BLAST analysis was used to suggest reactivity with VlsE from B. burgdorferi sources based on 100% homology with the immunizing sequence. Cross-reactivity with VlsE from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Ohnishi J, Schneider B, Messer WB, Piesman J, and de Silva AM. (2003) Genetic Variation at the vlsE Locus of Borrelia burgdorferi within Ticks and Mice over the Course of a Single Transmission Cycle. J Bacteriol. 185(15): 4432-4441. Magnarelli LA, Lawrenz M, Norris SJ, and Fikrig E. (2002) Comparative reactivity of human sera to recombinant VlsE and other Borrelia burgdorferi antigens in class-specific enzyme-linked immunosorbent assays for Lyme borreliosis. J. Med. Microbiol. (51) 649-655 Kornacki JA and Oliver DB. (1998) Lyme Disease-Causing Borrelia Species Encode Multiple Lipoproteins Homologous to Peptide-Binding Proteins of ABC-Type Transporters. Infection and Immunity 66(9): 4115-4122
Specific Reference
Log in to submit your product review.

Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-301

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-319

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
111-1102

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
111-3102

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry