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Primary Antibodies  >  Sample Size Primary Antibodies
 

Cenexin-1 Antibody

Rabbit Polyclonal
Code

200-401-C43S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Anti-Cenexin-1 in Western Blot using Rockland Immunochemical's Protein A Purified Anti-Cenexin-1 antibody shows detection of Cenexin-1 in total cell lysates from mouse F9 embryonic carcinoma cells.  Arrowheads show detection of Cenexin-1 at approximately 93kDa and ODF2 at approximately 70kDa. In personal communication with K. Lee, CCR-NCI, Bethesda, MD.
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Synonyms: ODF84 antibody, Outer dense fiber of sperm tail 2 antibody, Cenexin1 variant 1 antibody, KKT 4 antibody

Cenexin-1 Antibody Properties

Description
Anti-Cenexin-1 (Rabbit) Antibody - 200-401-C43S
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:500-1:10,000
Western Blot : 1:500-1:2000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.53 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
ODF2
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Lot#  

Cenexin-1 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cenexin-1 (ODF84) and cenexin-2 are splicing variants that are expressed from the same genetic locus Outer Dense Fiber protein 2 (ODF2) . Cenexin-1 and cenexin-2, regarded as centriolar proteins. Cenexin-1, a 93-kD variant of ODF2, is the major form of cennexin expressed in various somatic cells and tissues and associates with centrosomes. Cenexin-1 contains a unique C-terminal extension that appears to play a critical role in recruiting mitotic regulators such as polo-like kinase 1. In contrast, ODF2, a 70-kDa protein, is a major component of sperm tail cytoskeleton and abundantly expressed in testis. Additional evidence suggests that both of these proteins play important role in primary cilia formation. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation.  Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene; however, not all isoforms and variants have been fully described.
Immunogen
Anti-Cenexin-1 purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a truncated recombinant protein hCenexin1.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 93 kDa in size corresponding to Cenexin-1 by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This antibody was purified from monospecific antiserum by protein-A purified immunoaffinity chromatography. It is directed against, and shows specific reactivity for, human Cenexin-1 protein. A BLAST analysis was used to suggest cross-reactivity with Cenexin-1 from human, chimpanzee, mouse, rat, bovine sources based on 100% homology with the immunizing sequence. Reactivity with Cenexin-1 protein from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Soung,N.K., Kang,Y.H., Kim,K., Kamijo,K., Yoon,H., Seong,Y.S., Kuo,Y.L., Miki,T., Kim,S.R., Kuriyama,R., Giam,C.Z., Ahn,C.H. and Lee,K.S. (2006) Requirement of hCenexin for proper mitotic functions of polo-like kinase 1 at the centrosomes. Mol. Cell. Biol. 26 (22), 8316-8335. Kierszenbaum,A.L. (2002) Keratins: unraveling the coordinated construction of scaffolds in spermatogenic cells. Mol. Reprod. Dev. 61 (1), 1-2. Petersen,C., Fuzesi,L. and Hoyer-Fender,S. (1999) Outer dense fibre proteins from human sperm tail: molecular cloning and expression analyses of two cDNA transcripts encoding proteins of approximately 70 kDa.  Mol. Hum. Reprod. 5 (7), 627-635.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
200-401-915

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
600-401-916

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, chimpanzee, macaque
Catalog Number
600-401-A46

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, chimpanzee, macaque
Catalog Number
600-401-A47

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry