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Primary Antibodies  >  Neuroscience Antibodies
 

Tau Antibody

Rabbit Monoclonal SP70 IgG
Code

200-C01-B33
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Rockland's anti-tau monoclonal antibody (Rabbit) was used to detect Tau in human brain.  Tissue was formalin-fixed and paraffin embedded.  Staining requires boiling of sections in 10 mM citrate  buffer pH 6.0 for 10 min followed by cooling at RT  for 20 min. The primary antibody was diluted 1:50 and reacted with tissue for 30 min at RT.
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Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Microtubule-associated protein tau Neurofibrillary tangle protein Paired helical filament-tau

Tau Antibody Properties

Description
Anti-Tau (RABBIT) Monoclonal Antibody - 200-C01-B33
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human
Clonality
Monoclonal SP70 IgG
Application
ELISA : 1:20,000
Western Blot : 1:500-1:2000
Immunochemistry: 1:50
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG
Label
Concentration
N/A
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
MAPT
Download CofA
Lot#  

Tau Antibody Description

Background
The MAPT gene encodes the microtubule-associated protein Tau whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. Tau promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that Tau functions as a linker protein between both. Axonal polarity is predetermined by Tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of Tau.
Immunogen
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to the N-terminus of human Tau protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.
Immunogen Type
Peptide
Storage Condition
This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Application Note
This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to Tau protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.
Purity/Specifity
This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human Tau protein. Cross-reactivity with Tau protein from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Goedert M., Wischik C., Crowther R., Walker J., Klug A. (1988) Cloning and sequencing of the cDNA encoding a core protein of the paired helical filament of Alzheimer disease: identification as the microtubule-associated protein Tau. Proc. Natl. Acad. Sci. U.S.A. 85:4051-4055. Goedert M., Spillantini M.G., Potier M.-C., Ulrich J., Crowther R.A. (1989) Cloning and sequencing of the cDNA encoding an isoform of microtubule-associated protein Tau containing four tandem repeats: differential expression of Tau protein mRNAs in human brain. EMBO J. 8:393-399. Lee G., Neve R.L., Kosik K.S. (1989) The microtubule binding domain of Tau protein. Neuron 2:1615-1624. Goedert M., Spillantini M.G., Jakes R., Rutherford D., Crowther R.A. (1989) Multiple isoforms of human microtubule-associated protein Tau: sequences and localization in neurofibrillary tangles of Alzheimer's disease. Neuron 3:519-526. Andreadis A., Brown W.M., Kosik K.S. (1992) Structure and novel exons of the human Tau gene. Biochemistry 31:10626-10633.
Specific Reference
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Product Type
Controls;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat. chicken, bovine
Catalog Number
200-301-880

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat. chicken, bovine
Catalog Number
600-401-880

Product Type
Controls;
Applications
ELISA, WB, IHC
Reacts With
Mouse, Rat, Human, Monkey, Sheep, Bovine, Goat, Guinea Pig, Swine, Hamster
Catalog Number
600-403-886

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry