14-12_peptide top banner
 
Primary Antibodies  >  Apoptosis Antibodies
 

BAX Antibody

Rabbit Monoclonal SP47 IgG
NCI Collaboration
Order Offline
Rockland's anti-BAX monoclonal antibody (Rabbit) was used to detect BAX in normal human breast tissue. Tissue was formalin-fixed and paraffin embedded.  Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.  The primary antibody was diluted 1:50 and reacted with tissue for 30 min at RT.
View All Images
Number:
200-C01-B34
Size:
100 µL
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

BAX Antibody Properties

Description
Anti-BAX (RABBIT) Monoclonal Antibody - 200-C01-B34
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human
Clonality
Monoclonal SP47 IgG
Application
ELISA : 1:20,000
Flow Cytometry : 1:100
Western Blot : 1:500 – 1:2,000
Immunohistochemistry: 1:50
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgG
Label
Concentration
N/A
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
BAX
Download CofA:
Lot#  
Print

BAX Antibody Description

Background
BAX, also known as Bcl-2-like protein 4 and BCL2L4 is known to accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. BAX induces the release of cytochrome c, activation of CASP3, and thereby apoptosis. BAX protein is expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histyocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Isoform alpha is considered to be the 'canonical' sequence.
Synonyms
BAX Bcl-2-like protein 4 BCL2L4
Immunogen
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human BAX protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.
Immunogen Type
Peptide
Storage Condition
Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. Contains 1% BSA.
Application Note
This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to BAX protein by western blotting in the appropriate cell lysate or extract. BAX is found in normal breast tissue as a cytoplasmic protein and also at the cell membrane. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.
Purity/Specifity
This product was purified from concentrated tissue culture supernate by Protein A/G chromatography. This antibody is specific for human BAX protein. Cross-reactivity with BAX protein from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Oltvai Z.N., Milliman C.L., Korsmeyer S.J.(1993) Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74:609-619. Apte S.S., Mattei M.-G., Olsen B.R. (1995) Mapping of the human BAX gene to chromosome 19q13.3-q13.4 and isolation of a novel alternatively spliced transcript, BAX delta. Genomics 26:592-594. Shi B., Triebe D., Kajiji S., Iwata K.K., Bruskin A., Mahajna J. (1999) Identification and characterization of baxepsilon, a novel bax variant missing the BH2 and the transmembrane domains. Biochem. Biophys. Res. Commun. 254:779-785. Schmitt E., Paquet C., Beauchemin M., Dever-Bertrand J., Bertrand R. (2000) Characterization of Bax-sigma, a cell death-inducing isoform of Bax. Biochem. Biophys. Res. Commun. 270:868-879.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat, xenopus
Catalog Number
200-301-964

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
200-401-A34

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
Human, Mouse, Rat
Catalog Number
600-401-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, mouse, rat
Catalog Number
600-401-966

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry