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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-2 Antibody

Rabbit Polyclonal
Code

202-401-B95
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's protein-A purified anti-Cat IL-2 antibody shows detection of recombinant Cat IL-2 at 15.4 kDa, raised in yeast.. Primary antibody was diluted to 1 µg/ml. 3% BSA from Rockland's BSA-30 (Bovine Serum Albumin Solution) was used for blocking. Secondary antibody 611-131-122 (Goat anti-Rabbit IgG IRDye® 800) was used at 1:20,000.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Interleukin-2, IL-2, IL2, T-cell growth factor, TCGF, Aldesleukin, Lymphokine

IL-2 Antibody Properties

Description
Anti-Cat IL-2 (RABBIT) Antibody - 202-401-B95
Host
Rabbit
Target Species
Cat
Known Cross Reactivity
Cat
Clonality
Polyclonal
Application
ELISA : 1:20,000
Western Blot : 1:500-1:2,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
IL2
Download CofA
Lot#  

IL-2 Antibody Description

Background
IL-2 is a member of a family of cytokines, which also includes IL-4, IL-7, IL-9, IL-15 and IL-21. All members of this family signal through a receptor complex that includes the common gamma chain (?c). IL-2 signals through a receptor complex consisting of IL-2 specific IL-2 receptor alpha (CD25), IL-2 receptor beta (CD122) and the common gamma chain (?c). The main function of IL-2 is to promote the development of T regulatory cells. IL-2 is produced by several cell types including, B cells, ?d T cells, CD4+ T cells, CD8+ T cells, and eosinophils. It has been found to be similar to IL-15 in terms of function. Both IL-2 and IL-15 are able to facilitate production of immunoglobulins made by B cells and induce the differentiation and proliferation of natural killer cells. The primary differences between IL-2 and IL-15 are found in adaptive immune responses: IL-2 participates in maintenance of T-Regs and reduces self-reactive T cells while IL-15 is necessary for maintaining highly specific T cell responses by supporting survival of CD8 memory T cells.
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by by repeated immunizations with a recombinant protein raised in yeast corresponding to 134 amino acids of mature cat IL-2.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.4 kDa in size corresponding to feline IL-2 by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was protein A purified from monospecific antiserum by chromatography. This antibody is specific for cat IL-2 protein. A BLAST analysis was used to suggest cross-reactivity with IL-2 from cat based on 100% homology; cross-reactivity with Northern elephant seal and gray seal based on 90% homology with the immunizing sequence. Cross-reactivity with IL-2 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Gillis S., Ferm M., Ou W., Smith KA. (1978). T cell growth factor: Parameters of production and a quantitative microassay for activity. J. Immunol. 120 (6): 2027-2032. Sakaguchi S., Sakaguchi N., Asano M., Itoh M., Toda M. (1995). Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases. J. Immunol. 155 (3): 1151-64.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
Human
Catalog Number
109-401-311

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Cat
Catalog Number
202-401-311

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Human
Catalog Number
209-401-B95

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry