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Primary Antibodies  >  Infectious Disease Antibodies
 

Interferon Gamma Antibody

Rabbit Polyclonal
Code

203-401-C41
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's protein-A purified anti-chicken IFN gamma antibody shows detection of recombinant chicken IFN gamma at 16.7 kDa, raised in yeast.   Primary antibody was diluted to 1µg/mL.  3% BSA from Rockland’s BSA-30 (Bovine Serum Albumin Solution) was used for blocking.  Secondary antibody 611-131-122 (Goat anti-Rabbit IgG IRDye 800) was used at 1:20,000.
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Synonyms: Interferon gamma, IFNG, IFN

Interferon Gamma Antibody Properties

Description
Anti-Chicken Interferon Gamma (RABBIT) Antibody - 203-401-C41
Host
Rabbit
Target Species
Chicken
Known Cross Reactivity
Chicken
Clonality
Polyclonal
Application
ELISA : 1:20,000
Western Blot : 1:500-1:2,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
IFN gamma
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Interferon Gamma Antibody Description

Background
Interferon-gamma (IFN-gamma) is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-gamma belongs. IFN-gamma, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-gamma in the immune system stems in part from its ability to inhibit viral replication directly, but, most important, derives from its immunostimulatory and immunomodulatory effects. IFN-gamma is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 145 amino acids of mature chicken IFN gamma.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.7 kDa in size corresponding to chicken IFN gamma by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was protein A purified from monospecific antiserum by chromotography. This antibody is specific for chicken IFN gamma protein. A BLAST analysis was used to suggest cross-reactivity with IFN gamma from chicken based on 100% homology; ring-necked pheasant, turkey, quail, and junglefowl based on 91-97% homology with the immunizing sequence. Cross-reactivity with IFN gamma from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Schroder K., Hertzog PJ., Ravasi T., Hume DA. (2004). Interferon-gamma: an overview of signals, mechanisms and functions. J. Leukoc. Biol. 75 (2): 163-89. Ikeda H., Old LJ., Schreiber RD. (2002). The roles of IFN gamma in protection against tumor development and cancer immunoediting. Cytokine Growth Factor. Rev. 13 (2): 95-109.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Bovine
Catalog Number
201-401-C41

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
203-4302

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-C64

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-C64S

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry