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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-1 beta Antibody

Rabbit Polyclonal
Code

209-401-301
Size

1 mg
Price

$309.00
Availability

Ships next business day.
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Immunohistochemistry of HumanIL1beta_antibod

Tissue: medullary lymph node

Fixation:  formalin fixed paraffin embedded  

Antigen retrieval:  user optimized

Primary antibody:  Human IL1beta antibody

Secondary antibody:  Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT

Localization:  cytoplasm

Staining: Close up of medullary lymph node: positive staining in the cytoplasm of circulating macrophages.  Neg Ctr (far right) normal  rabbit IgG with pH 6.2    40X
IL-1B antibody was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1B by Western blot.  Lane 1 and 2 were loaded with 2.5 ?g and 500 ng of dog IL-1B respectively.  The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown).  Detection occurred using a 1:3,000 dilution of IRDye™800 conjugated Donkey anti-Rabbit IgG (code # 611-732-127) for 1h at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.  Other detection systems will yield similar results.
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Synonyms: IL-1 beta, Interleukin-1 beta, IL-1ß, catabolin

IL-1 beta Antibody Properties

Description
Anti-Human IL-1ß (RABBIT) Antibody - 209-401-301
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, primate, dog
Clonality
Polyclonal
Application
ELISA : 1:500 - 1:2,000
Neutralization : 1:100
Western Blot : 1:1,000
Immunochemistry: 1:100 - 1:200
ImmunoPrecipitation: 1:400 - 1:800
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
2.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
None
Stabilizer
None
GeneName
IL1B
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Lot#  

IL-1 beta Antibody Description

Background
IL-1 beta (also known as Interleukin-1 beta, IL-1ß and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1ß is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
Immunogen
This antibody was prepared by repeated immunizations with recombinant human IL-1ß produced in E.coli.  The MW of the recombinant 153 aa IL-1ß was 17 kDa with the N-terminal amino acid at position alanine 117.  This cleavage site is generated by the IL-1ß converting enzyme (ICE, capase-1).
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Human IL-1ß has been tested for use in neutralizations, ELISA, radioimmunoassays, flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17,000 MW mature IL-1ß. For immunoblots, typically, IL-1ß is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG (p/n 011-001-297) to reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL-1ß. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (611-1302) for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of human and primate IL-1ß activity in bioassays. It does not neutralize the biological activity IL-1a. It does not neutralize the biological activity of murine, rat or rabbit IL-1ß. For neutralization, it is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.
Purity/Specifity
This is an IgG preparation of whole rabbit serum purified by DEAE fractionation. This antibody is primarily directed against mature, 17,000 MW human IL-1ß and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1ß in the same formats using similar dilutions. The antiserum does not recognize human IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1ß containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1ß precursor molecule. Denatured 31,000 precursor IL-1ß will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1ß exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, the antibody detects the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1ß precursor in lysates.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Auron, P.E., A.C. Webb, et al (1984) Nucleotide sequence of human monocyte interleukin 1 precursor cDNA. Proc Natl Acad Sci USA 81: 7907-7911. Cerretti, D.P., C.J. Kozlosky, et al. (1992) Molecular cloning of the IL-1ßprocessing enzyme. Science 256: 97-100. Herzyk, D.J., A.E. Berger, et al. (1992) Sandwich ELISA formats designed to detect 17 kDa IL-1ßsignigicantly underestimate 35 kDa IL-1ß J Immunol Meth 148: 243 -254.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP, Neu
Reacts With
human, primate, dog
Catalog Number
109-401-301

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
109-401-312

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-401-302

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse
Catalog Number
600-401-955

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry