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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-1 Alpha Antibody

Rabbit Polyclonal
Code

209-401-302
Size

1 mg
Price

$309.00
Availability

Ships next business day.
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Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Interleukin 1 alpha precursor antibody, Interleukin1 alpha antibody, Preinterleukin 1 alpha antibody

IL-1 Alpha Antibody Properties

Description
Anti-Human IL-1 alpha (RABBIT) Antibody - 209-401-302
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:500 - 1:2,000
Immunochemistry: 1:100 to 1:200
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
IL1A
Download CofA
Lot#  

IL-1 Alpha Antibody Description

Background
Interleukin 1 alpha is produced by activated macrophages. Anti-IL-1 alpha antibody is ideal for investigators involved in Immunology research.
Immunogen
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli.  The MW of the recombinant IL-1a was 17,000.  This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This IgG fraction antibody of anti-Human IL-1a has been tested for use in neutralizations, ELISA,  immunohistochemistry, flow cytometry and immunoblotting.  It recognizes the 17,000 MW mature IL-1a.  Reactivity in other immunoassays is unknown.
Purity/Specifity
This is an IgG preparation of whole rabbit serum purified by DEAE fractionation.  This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays.  In general, this antibody also detects primate IL-1a in the same formats using similar dilutions.  The antiserum does not recognize human IL-1b or Mouse or Rabbit IL-1a.  In ELISA formats and other immunoreactive assays, this antibody will recognize both the mature 17,000 MW IL-1a as well as the 31,000 MW IL-1a precursor in either non-denatured (native) or denatured samples.  Unlike the IL-1b precursor, the native precursor of IL-1a is recognized by the antibody produced to the 17,000 MW form.   The 31,000 precursor of IL-1a is biologically active and is found primarily intracellularly.  The precursor of IL-1a, unlike that of IL-1b, is biologically active when applied to cells and is thought to have a role as a functional molecule intracellularly and can be found constitutively expressed in various cell.  This antibody is also useful for neutralization of human and primate IL-1a activity in bioassays.  It does not neutralize the biological activity IL-1b.  It does not neutralize the biological activity of mouse, rat or rabbit IL-1a.  It will neutralize primate IL-a.  For neutralization, it is recommended to incubate the sample with a 1:100 dilution of the antibody for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG is recommended.  This antibody can be used for FACS analysis.  Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Cerretti, D.P., et al. (1992) Molecular cloning of the IL-1alpha processing enzyme. Science 256: 97-100. Thornberry, N.A., et al. (1992) A novel heterodimeric cysteine protease is required for interleukin-1alpha processing in monocytes. Nature 356 (6372) 768-74. Lonnemann, G., et al. (1995) Cytokines in human renal interstitial fibrosis. II. Intrinsic Interleukin (IL)-1 synthesis and IL-1-dependent production of IL-6 and IL-8 by cultured kidney fibroblasts. Kidney Int 47: 845-854. Lonnemann, G., et al. (1995) Cytokines in juman renal interstitial fibrosis. I Interleukin-a is a paracrine growth factor for cultured fibrosis-derived kidney fibroblasts. Kidney Int 47: 837-844. Stevenson, F.T., et al. (1997) The N-terminal propiece of interleukin 1alpha is a transforming nuclear oncoprotein. Proc Natl Acad Sci USA 94: 508-513.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP, Neu
Reacts With
human, primate, dog
Catalog Number
109-401-301

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
109-401-312

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse
Catalog Number
600-401-955

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry