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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-6 Antibody

Rabbit Polyclonal
Code

209-401-310
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's anti-Human IL6 antibody shows detection of a band ~24 kDa in size corresponding to recombinant human IL6 (arrowhead). Molecular weight markers are also shown.  After transfer, the membrane was blocked overnight with 1% BSA in TTBS followed by reaction with primary antibody at a 1:500 dilution.  Detection occurred using HRP conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) and FemtoMax™ Chemiluminescent substrate (p/n FEMTOMAX-110).  Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
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Synonyms: HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF

IL-6 Antibody Properties

Description
Anti-Human IL-6 (RABBIT) Antibody - 209-401-310
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10,000
Neutralization : Yes
Western Blot : 1:300 - 1:1,000
Immunohistochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
IL6
Download CofA
Lot#  

IL-6 Antibody Description

Background
Anti-IL-6 antibody is validated by ELISA, Immunohistochemistry and Western Blot Assays. IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with mature length recombinant human IL-6 produced in E.coli.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This purified antibody has been tested for use in ELISA, IHC and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
IL-6 antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.  The antiserum is also useful for neutralization of human of IL-6 activity in bioassays.  For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.  In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6.  However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. This product has minimal reactivity with mouse IL-6.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Novick, D., et al. (1989) Monoclonal antibodies for affinity Purification of IL-6/IFNveta 2 and for neutralization of HGF activity. Hybridoma 8: 561-567. Novick, D., et al. (1992) Enhancement of interleukin 6 cytostatic effect on human breast carcinoma cells by soluble IL-6 receptor from urine and reversion by monoclonal antibody. Cytokine 4 (1): 6-11. Silvera, M.R., et al. (1994) Expression of TGFa isoforms by thy-1+ and Thy-1- pulmonary fibroblast subsets: evidence for TGFa as a regulator of IL-dependent stimulation of IL-6. Lymphokine Cytokine Res 13: 277-285
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-310

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human
Catalog Number
109-401-310

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
Human
Catalog Number
109-401-311

Product Type
Primary Antibodies;
Applications
ELISA, WB, FC
Reacts With
Human
Catalog Number
209-301-310

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry