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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-2 Antibody

Rabbit Polyclonal
Code

209-401-B95
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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E-mail: orders@rockland-inc.com
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Rockland's anti-Human IL-2 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-2. The identity of the faint higher molecular weight band may represent a homodimer.  Molecular weight markers are also shown (left).  After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution.  Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate.  Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Synonyms: Interleukin-2, IL-2, IL2, T-cell growth factor, TCGF, Aldesleukin, Lymphokine

IL-2 Antibody Properties

Description
Anti-Human IL-2 (RABBIT) Antibody - 209-401-B95
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human
Clonality
Polyclonal
Application
ELISA : 1:10,000
Western Blot : 1:1,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
IL2
Download CofA
Lot#  

IL-2 Antibody Description

Background
IL-2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. Anti-IL-2 antibody is ideal for investigators involved in Immunology research.
Immunogen
IL-2 antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-2 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store Anti-IL-2 antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 15 kDa in size corresponding to native human IL-2 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-2 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Bazan,J.F. (1992) Unraveling the structure of IL-2. Science 257 (5068), 410-413. Brodie,C. and Gelfand,E.W. (1992) Functional nerve growth factor receptors on human B lymphocytes. Interaction with IL-2. J. Immunol. 148 (11), 3492-3497. Waldmann,T.A. (1991) The interleukin-2 receptor. J. Biol. Chem. 266 (5), 2681-2684.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-B95

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Cat
Catalog Number
202-401-B95

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-403-B95

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-406-B95

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry