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Primary Antibodies > Cytokines & Growth Factor Antibodies

MIP-3 alpha Antibody Biotin Conjugated

Rabbit Polyclonal

Code

209-406-B51

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western Blot of Biotin Conjugated Rabbit Anti-Human MIP-3a Antibody.
Lane 1: Human MIP-3a.
Lane 2: None.
Load: 50 ng per lane.
Primary antibody: Human MIP-3a antibody at 1:1000 for 60 min at RT.
Secondary antibody: Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size: 10 kDa, 10 kDa for Human MIP-3a.
Other band(s): None.

Shipping & Handling Fees Apply.

Shipping:

$40.00 to United States

Handling:

$35.00

Synonyms: MIP-3a , CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1

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MIP-3 alpha Antibody Biotin Conjugated Properties

Description

Anti-Human MIP-3a (RABBIT) Antibody Biotin Conjugated - 209-406-B51

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:20,000-1:100,000
Western Blot : 1:2,000-1:10,000
Immunohistochemistry: 1:1,000-1:5,000

Physical State

Lyophilized

Shipping condition

Ambient

Format

IgG

Label

Biotin

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reconstitution Volume

100 µL

Reconstitution Buffer

Restore with deionized water (or equivalent)

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

GeneName

CCL20

MIP-3 alpha Antibody Biotin Conjugated Description

Background

MIP-3a (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3a inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3a also possesses antibacterial activity against E.coli and S.aureus. MIP-3a is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. Cancer, Cell Biology and Immunology Research

Immunogen

MIP-3 alpha Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3a protein.

Immunogen Type

Recombinant Protein

Storage Condition

Store MIP-3 alpha Antibody Biotin Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 11 kDa in size corresponding to human MIP-3a protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Purity/Specifity

Human MIP-3 alpha Antibody Biotin Conjugatedwas heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human MIP-3a in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Lisignoli,G., Manferdini,C., Codeluppi,K., Piacentini,A., Grassi,F., Cattini,L., Filardo,G. and Facchini,A. (2009) CCL20/CCR6 chemokine/receptor expression in bone tissue from osteoarthritis and rheumatoid arthritis patients: different response of osteoblasts in the two groups. J. Cell. Physiol. 221 (1), 154-160. Punj,V., Matta,H., Schamus,S., Yang,T., Chang,Y. and Chaudhary,P.M. (2009) Induction of CCL20 production by Kaposi sarcoma-associated herpesvirus: role of viral FLICE inhibitory protein K13-induced NF-kappaB activation. Blood 113 (22), 5660-5668. Reboldi,A., Coisne,C., Baumjohann,D., Benvenuto,F., Bottinelli,D., Lira,S., Uccelli,A., Lanzavecchia,A., Engelhardt,B. and Sallusto,F. (2009) C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE. Nat. Immunol. 10 (5), 514-523.

Specific Reference

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