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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-3 Antibody Biotin Conjugated

Rabbit Polyclonal
Code

209-406-B92
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western Blot of Rabbit anti-Human IL-3 Biotin Conjugated Antibody.  
Lane 1:  Human IL-3.  
Lane 2:  None.  
Load:  50 ng per lane.
Primary antibody:  Human IL-3 primary antibody at 1:1,000 overnight at 4°C.
Secondary antibody:  Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  15 kDa, 15 kDa for Human IL-3. 
Other band(s): None.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Burst promoting activity, Colony stimulating factor, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL 3, Interleukin 3

IL-3 Antibody Biotin Conjugated Properties

Description
Anti-Human IL-3 (RABBIT) Antibody Biotin Conjugated - 209-406-B92
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:20,000-1:100,000
Western Blot : 1:2,000-1:10,000
Immunohistochemistry: 1:1,000-1:5,000
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
IL3
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Lot#  

IL-3 Antibody Biotin Conjugated Description

Background
The pleiotropic IL3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.
Immunogen
Anti-Human IL-3 (RABBIT) Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store Anti-Human IL-3 (RABBIT) Antibody Biotin Conjugated at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 15 kDa in size corresponding to human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Biotin Conjugated Anti-Human IL-3 Antibody Biotin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-3 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Davila,S., Froeling,F.E., Tan,A., Bonnard,C., Boland,G.J., Snippe,H., Hibberd,M.L. and Seielstad,M. (2010) New genetic associations detected in a host response study to hepatitis B vaccine. Genes Immun. 11 (3), 232-238. Simmonds,M.J., Yesmin,K., Newby,P.R., Brand,O.J., Franklyn,J.A. and Gough,S.C. (2010) Confirmation of association of chromosome 5q31-33 with United Kingdom Caucasian Graves' disease. Thyroid 20 (4), 413-417. Zhu,W., Liu,N., Zhao,Y., Jia,H., Cui,B. and Ning,G. (2010) Association analysis of polymorphisms in IL-3, IL-4, IL-5, IL-9 and IL-13 with Graves' disease. J. Endocrinol. Invest. (2010) In press
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-B92

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-403-B92

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-406-B92

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, chimpanzee, mouse
Catalog Number
600-401-693

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester