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Primary Antibodies  >  Cytokines & Growth Factor Antibodies

IL-7 Antibody Biotin Conjugated

Rabbit Polyclonal


100 µg


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Rockland's anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer.  Molecular weight markers are also shown (left).  After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution.  Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate.  Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
Western Blot showing detection of Human IL-7. 50ng of Human IL-7 (Lane 1) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose.  After blocking with 5% Blotto (p/n B501-0500) 30 min at 20°C, Anti-Human IL-7 (RABBIT) Antibody Biotin Conjugated (p/n 209-406-B94) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070).  HRP Streptavidin (p/n S000-03) was used at 1:40,000 in MB-070 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP.  Arrow indicates correct 17 kDa molecular weight position expected for Human IL-7.
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Synonyms: IL7, IL-7 Interleukin 7, Interleukin-7

IL-7 Antibody Biotin Conjugated Properties

Anti-Human IL-7 (RABBIT) Antibody Biotin Conjugated - 209-406-B94
Target Species
Known Cross Reactivity
ELISA : 1:20,000-1:100,000
Western Blot : 1:2,000-1:10,000
Immunohistochemistry: 1:1,000-1:5,000
Other Dilution: User Optimized
Physical State
Shipping condition
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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IL-7 Antibody Biotin Conjugated Description

Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 17.4 kDa in size corresponding to human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-7 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Markley,J.C. and Sadelain,M. (2010) IL-7 and IL-21 are superior to IL-2 and IL-15 in promoting human T cell-mediated rejection of systemic lymphoma in immunodeficient mice. Blood 115 (17), 3508-3519. Henriques,C.M., Rino,J., Nibbs,R.J., Graham,G.J. and Barata,J.T. (2010) IL-7 induces rapid clathrin-mediated internalization and JAK3-dependent degradation of IL-7Ralpha in T cells. Blood 115 (16), 3269-3277. Bikker,A., van Woerkom,J.M., Kruize,A.A., Wenting-van Wijk,M., de Jager,W., Bijlsma,J.W., Lafeber,F.P. and van Roon,J.A. (2010) Increased expression of interleukin-7 in labial salivary glands of patients with primary Sjogren's syndrome correlates with increased inflammation. Arthritis Rheum. 62 (4), 969-977.
Specific Reference
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Proteins and Peptides;
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Primary Antibodies;
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Primary Antibodies;
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Product Type
Primary Antibodies;
Reacts With
human, mouse, rat
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Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester