Datasheet

Esrp-2 Antibody

Name: Esrp-2 Antibody
Brand: Rockland Immunochemicals
Price: 125.0 USD
Availability: InStock

Mouse Monoclonal 22C5.H7.A6 IgG2a kappa

1 References

Item No. 210-301-C32

Size: 100 µg

Applications: WB, ELISA

Reactivity: Mouse

Host Species: Mouse

$485.00 /Per Item

25 µL $125.00

100 µg $485.00

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Background

Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Expand Background

Specifications for Esrp-2 Antibody

Product Details

Description: Anti-Esrp-2 (MOUSE) Monoclonal Antibody - 210-301-C32

Synonyms: mouse anti-Esrp2 antibody, mouse anti-Esrp-2 antibody, RNA-binding protein 35B, RNA-binding motif protein 35B

Host Species: Mouse

Clonality: Monoclonal

Format: IgG2a

Target Details

Gene Name: Esrp2 - View All Esrp2 Products

Reactivity: Mouse

Immunogen Type: Recombinant Protein

Immunogen: Anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.

Purity/Specificity: Anti-Esrp-2 is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with mouse Esrp-2 protein. A BLAST analysis was used to suggest cross-reactivity with Esrp-2 from mouse based on a 100% homology with the immunizing sequence. Cross-reactivity with Esrp-2 from other sources has not been determined.

Database Links:

UniProtKB - Q8K0G8

NCBI - NCBI

GeneID - 77411

Application Details

Tested Applications: ELISA, WB

Application Note: Anti-Esrp-2 protein A purified antibody has been tested for use western blotting and ELISA. This antibody shows specific reactivity by with ESRP2 transfected cell lysates. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (1)

Heilmann et al. (2017). Genome-wide screen for differentially methylated long noncoding RNAs identifies Esrp2 and lncRNA Esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer. Oncogene

Applications

WB, IB, PCA

PubMed

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.