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IL-6 Mouse Recombinant Protein - 010-001-310
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Anti-Human IL-6 (RABBIT) Antibody - 209-401-310
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Anti-Mouse IL-6 (RABBIT) Antibody - 210-401-310
Anti-Mouse IL-6 (RABBIT) Antibody - 210-401-310
Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL6 Antibody

Rabbit Polyclonal
Code

210-401-310
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Anti-mouse IL-6 antibody in western blot shows detection of recombinant mouse IL-6 raised in E.coli.  Recombinant truncated protein (0.1 µg, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Rockland's Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C.  After washes, the blot was reacted with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. at RT.  Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
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Synonyms: Interleukin-6 cytokine, IL-6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, IFN-beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, Interleukin HP-1

IL6 Antibody Properties

Description
Anti-Mouse IL-6 (RABBIT) Antibody - 210-401-310
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Polyclonal
Application
ELISA : 1:10,000
Western Blot : 1:1000
Immunohistochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
Il-6
Download CofA
Lot#  

IL6 Antibody Description

Background
Interleukin (IL)-6, also know as BCDG, BCGF and BSF-2, is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Anti-IL-6 antibody is ideal for investigators involved in Cancer, Neuroscience and Immunology research.
Immunogen
Anti-IL-6 is an IgG fraction antibody prepared from rabbit antiserum after repeated immunizations with recombinant mouse IL-6 protein produced in E.coli.
Immunogen Type
Recombinant Protein
Storage Condition
Store anti-IL-6 at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21.7 kDa in size corresponding to the mature 187 amino acid mouse IL-6 protein by western blotting in appropriate cell lysate or extract.
Purity/Specifity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This antibody is specific for mouse IL-6 protein. A BLAST analysis was used to suggest cross-reactivity with IL-6 from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-6 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Blankenstein T., Qin Z., Li W., Diamantstein T. (1990) DNA rearrangement and constitutive expression of the interleukin 6 gene in a mouse plasmacytoma. J. Exp. Med. 171:965-970 [PubMed: 2106569] [Abstract]
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-310

Product Type
Primary Antibodies;
Applications
ELISA, WB, FC
Reacts With
Human
Catalog Number
209-301-310

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-401-310

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Mouse
Catalog Number
210-401-310

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry