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IL-35 EBI3 Antibody Peroxidase Conjugated

Rabbit Polyclonal
NCI Collaboration
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Western Blot of Rabbit anti-Mouse EBI-3 Antibody Peroxidase Conjugated.  
Lane 1:  Mouse EBI-3 Recombinant Protein.  
Load:  100 µg per lane.
Primary antibody:  Rabbit anti-Mouse EBI-3 Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C.
Secondary antibody: n/a.
Block:  MB-070 for 1 HR at RT.
Predicted/Observed size:  25 kDa, 27 kDa for Mouse EBI-3.
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100 µg
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IL-35 EBI3 Antibody Peroxidase Conjugated Properties

Anti-Mouse EBI-3 (RABBIT) Antibody Peroxidase Conjugated - 210-403-B66
Target Species
Known Cross Reactivity
Mouse, rat
ELISA : 1:10,000-1:50,000
Western Blot : 1:1,000-1:5,000
Immunohistochemistry: 1:500-1:2,500
Physical State
Shipping Condition
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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IL-35 EBI3 Antibody Peroxidase Conjugated Description

The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer.
Epstein-Barr virus induced 3 protein, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35, HRP
Anti-EBI-3 / IL27 beta Antibody Peroxidase Conjugate was prepared by repeated immunizations with recombinant mouse EBI3.
Immunogen Type
Recombinant Protein
Storage Condition
Store Anti-EBI-3 / IL27 beta Antibody Peroxidase Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kDa in size corresponding to the mature mouse EBI3 protein by western blotting in appropriate cell lysate or extract.
Anti-EBI-3 / IL27 beta Antibody Peroxidase Conjugate is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This antibody is specific for mouse and rat EBI-3 protein. Cross reactivity does NOT occur with human EBI-3. Cross-reactivity with IL-27 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Pflanz S., Timans J.C., Cheung J., Rosales R., Kanzler H., Gilbert J., Hibbert L., Churakova T., Travis M., Vaisberg E., Blumenschein W.M., Mattson J.D., Wagner J.L., To W., Zurawski S., McClanahan T.K., Gorman D.M., Bazan J.F. Kastelein R.A (2002). IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naive CD4(+) T cells. Immunity 16:779-790 [PubMed: 12121660]
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Catalog Number

Product Type
Primary Antibodies;
Reacts With
Mouse, rat
Catalog Number

Product Type
Primary Antibodies;
Reacts With
Mouse, rat
Catalog Number

Product Type
Supporting Reagents;
Catalog Number

Product Label


Peroxidase (Horseradish)
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
Excitation Wavelength
Conjugation Chemistry