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Blood Products  >  Antisera to Red Blood Cells
 

Sheep Red Blood Cell RBC Antibody

Rabbit Polyclonal
Code

213-4139
Size

50 mg
Price

$279.00
Availability

Ships next business day.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Anti-RBC antibody, Red Blood Cell Antibody, Antibody for hemagglutination, rabbit anti RBC, rabbit antibody to sheep Red Blood Cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent, anti-erythrocyte, anti-erythrocyte antibody

Sheep Red Blood Cell RBC Antibody Properties

Description
Anti-SHEEP Red Blood Cell (RBC) (RABBIT) Antibody - 213-4139
Host
Rabbit
Target Species
Sheep
Clonality
Polyclonal
Application
Other Dilution: AGGLUTINATION TITER 1:32 to 1:64
Physical State
Lyophilized
Shipping condition
Ambient
Product Type
Red Blood Cells
Sterility
Non-sterile
Format
IgG
Label
Concentration
10.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
5.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Sheep Red Blood Cell RBC Antibody Description

Background
Anti-SHEEP Red Blood Cell Antibody may be used in hemagglutination assays. Anti-SHEEP Red Blood Cell Antibody is used to sensitize erythrocytes and quantitate agglutination. Haemagglutination assay or HA is a method of quantification for viruses or bacteria by hemagglutination. Some viral families and many bacteria have envelope or surface proteins which are able to agglutinate (stick to) human or animal red blood cells (RBC) and bind to N-acetylneuraminic acid. As each of the agglutinating molecule attaches to multiple RBCs, a lattice-structure will form. Normally, a virus dilution (e.g. 2-fold from 1:4 to 1:4096) will be applied to an RBC dilution (e.g. 0.1% to 0.7% in steps of 0.2%) for approx. 30 min, often at 4° C, otherwise viruses with neuraminidase activity will detach the virus from the RBCs. Then the lattice forming parts will be counted and the titer calculated. The titer of a hemagglutination assay is determined by the last viable "lattice" structure found. This is because it is at the point where, if diluted anymore, the amount of Virus particles will be less than that of the RBCs and thus not be able to agglutinate them together.
Sterlization
Non-sterile
Immunogen
Sheep washed pooled Red Blood Cells (RBC)
Immunogen Type
Other
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
Suitable for agglutination of cells on titer plates. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Purity/Specifity
This product is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Blood Products;
Catalog Number
113-4139

Product Type
Blood Products;
Catalog Number
C200-0005

Product Type
Blood Products;
Catalog Number
C300-0050

Product Type
Blood Products;
Catalog Number
R311-0050

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry