F(ab')2 Chicken IgG (H&L) Antibody Peroxidase Conjugated

Datasheet

F(ab')2 Chicken IgG (H&L) Antibody Peroxidase Conjugated

Name: F(ab')2 Chicken IgG (H&L) Antibody Peroxidase Conjugated
Brand: Rockland Immunochemicals
Price: 325.0 USD
Availability: InStock

Rabbit Polyclonal

Item No. 303-4302

Size: 20 mg

Reactivity: Chicken

Host Species: Rabbit

$325.00 /Per Item

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Background

F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining.

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Specifications for F(ab')2 Chicken IgG (H&L) Antibody Peroxidase Conjugated

Product Details

Description: Rabbit F(ab')2 Anti-Chicken IgG (H&L) Antibody Peroxidase Conjugated - 303-4302

Synonyms: Rabbit F(ab')2 anti-Chicken IgG Antibody peroxidase Conjugated, Rabbit F(ab')2 anti-Chicken IgG HRP conjugated antibody, Rabbit F(ab')2 anti-Chicken IgY Antibody peroxidase Conjugated, Rabbit F(ab')2 anti-Chicken IgY HRP conjugated antibody

Host Species: Rabbit

Specificity: IgG (H&L)

Conjugate: Peroxidase (HRP)

Clonality: Polyclonal

Format: IgG F(ab')2

Target Details

Reactivity: Chicken

Immunogen: Chicken IgG whole molecule

Purity/Specificity: This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.

Application Details

Application Note: F(ab')2 Chicken IgG Antibody Peroxidase Conjugated is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Formulation

Physical State: Lyophilized

Concentration: 10.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.