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Primary Antibodies  >  Apolipoprotein Antibodies
 

APOLIPOPROTEIN A-I Antibody

Goat Polyclonal
Code

600-101-109
Size

1 mg
Price

$294.00
Availability

Ships next business day.
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Coommassie stained gel showing both free and HDL bound apoA-I eluted from a solid phase resin prepared using Rockland's anti-Human apolipoprotein A-I antibody.  The resin was reacted with human serum prior to washing and elution of bound proteins.  The gel was composed of 0.75% agarose in a native buffer system.  Separation occurred at room temperature.
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Synonyms: APOA1 antibody, APOA1/APOC3 fusion gene antibody, Apolipoprotein A I precursor antibody, Apolipoprotein AI antibody, Apolipoprotein of high density lipoprotein antibody

APOLIPOPROTEIN A-I Antibody Properties

Description
Anti-Apolipoprotein A-I (GOAT) Antibody - 600-101-109
Host
Goat
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:20,000
Western Blot : 1:1,000 - 1:2,000
Immunohistochemistry: 1:50 - 1:200
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
APOA1
Download CofA
Lot#  

APOLIPOPROTEIN A-I Antibody Description

Background
Anti Apolipoprotein A-I antibody recognizes the gene product of APOA1. Apolipoprotein promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18-amino acid signal peptide is removed co-translationally and a 6-amino acid propeptide is cleaved post-translationally. Variation in the latter step, in addition to modifications leading to so-called isoforms, is responsible for some of the polymorphism observed. APOA1 is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the C-III gene is transcribed convergently in relation to A-I. Defects in the apolipoprotein A-1 gene are associated with HDL deficiency and Tangier disease.
Immunogen
apoLipoprotein Type A-I was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to opening.   This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Application Note
Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation, immunohistochemistry and for western blotting for highly sensitive qualitative analysis.
Purity/Specifity
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.  Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.  This antibody reacts with human apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins.  Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined.  Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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AMCA Conjugates
Antigen Retrieval Methods
Azide Removal by Dialysis
Beta Galactosidase Conjugates
Biotin Binding Assay (HABA)
Biotin Conjugates
Cell Lysates Protocol
CyDye™ Conjugates
DyLight™ Antibody Conjugate Properties
DyLight™ Antibody Spectra
ELISA
ELISA to Quantify Collagen
Extended Conjugate Information Sheet
Fab Fragment Monovalent Antibodies
FACS / Flow Cytometry Analysis
Fluorescein Conjugates
Gel Shift Assay
General Antibody Information
Histone Immunoblotting
Immunoblotting
Immunocytochemistry
Immunofluorescence Microscopy
Immunofluorescence Microscopy - Assay Formats
Immunofluorescence Microscopy - ATTO-TEC
Immunofluorescence Microscopy - CyDye®
Immunofluorescence Microscopy - DyLight™
Immunofluorescence Microscopy - Fluorescent Labels
Immunofluorescence Microscopy - IRDye®
Immunofluorescence Microscopy - long
Immunofluorescence Microscopy - Microscope Types
Immunofluorescence Microscopy - Other Conjugates
Immunofluorescence Microscopy Protocol
Immunohistochemistry
Immunoprecipitation
Nuclear/Cytoplasmic Extract Isolation
Peroxidase & Alkaline Phosphatase
Phosphorylated State Proteins
Phosphorylated State Proteins - AKTs
Phosphorylated State Proteins - Long
Phosphorylated State Proteins - Pathways
Phosphorylated State Proteins - Phospho Specific
Phosphorylated State Proteins - Tailored Phosphorylated Target
Phosphorylated State Proteins - Western Blot
Phycoerythrin Conjugates
Protein A / Protein G Binding Reactivities
Recommended Dilutions
Rhodamine & Texas Red™
SDS-PAGE
Staining Paraffin Sections by PAP Procedure
USDA & Material Safety Notice
Western Blot - Buffers
Western Blot - Long
Western Blot - Preassembled Kits
Western Blot - Primary Antibody Reagents
Western Blot - Secondary Antibody Reagents
Western Blot - Substrates
Western Blot - Types of Blot Formats
Western Blot (Protein Immunoblot)
Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-101-109

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-101-110

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-101-111

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-101-112

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry