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Primary Antibodies  >  Neuroscience Antibodies
 

Beta Amyloid Antibody

Rabbit Polyclonal
Code

600-401-253
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Immunohistochemistical detection of beta Amyloid using Anti-Beta Amyloid Antibody on TG APP23 mouse brain cortex frozen sections. Anti-Beta Amyloid Antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. 
Carl Hobbs, King`s College London, United Kingdom
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Shipping:
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$20.00
Synonyms: Beta amyloid, A-beta

Beta Amyloid Antibody Properties

Description
Anti-Beta Amyloid (RABBIT) Antibody - 600-401-253
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
IF Microscopy : 1:50-1:200
Western Blot : 1:1000-1:5000
Immunochemistry: 1:50-1:200
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.99mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
APP
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Lot#  

Beta Amyloid Antibody Description

Background
Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques.  While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease.  In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid.  Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid.  Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.
Immunogen
This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the amino terminus (aa 1-14) of human beta amyloid conjugated to KLH using maleimide.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Affinity purified anti-beta amyloid detects beta amyloid in ELISA, IHC and IF. A 40-50 kD band consistent with a higher MW precursor is detected in western blot using whole tissue extracts from mouse brain.  In general, we recommend the use of 4% PFA for paraffin embedded tissues and 10% formalin for frozen tissue for fixation.
Purity/Specifity
This affinity-purified antibody is directed against the amino terminal end of beta amyloid and is useful in determining its presence in various assays.  Polyclonal anti-beta amyloid detects human and mouse beta amyloid.  Cross reactivity with beta amyloid from other species is likely but has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
N-terminal EFRH sequence of Alzheimer's beta-amyloid peptide represents the epitope of its anti-aggregating antibodies. (Frenkel D; J Neuroimmunol, 1998 Aug 1) Activation of nuclear factor-kappa B by beta-amyloid peptides and interferon-gamma in murine microglia. (Bonaiuto C; J Neuroimmunol, 1997 Jul) (Luo Y; Neurosci Lett, 1996 Oct 18) Alzheimer's beta-amyloid peptides induce inflammatory cascade in human vascular cells: the roles of cytokines and CD40. (Suo Z; Brain Res, 1998 Oct 5)
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
200-401-984

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry