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HA Epitope Tag Antibody
Rabbit Polyclonal
57 References
600-401-384S
600-401-384
25 µL
100 µg
WB, ELISA, IHC, IF, IP, Multiplex
HA-Tag
Rabbit
Availability: Ships next business day
Availability: Ships next business day
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Background
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.
Product Details
Anti-HA Epitope Tag (RABBIT) Antibody - 600-401-384
rabbit anti-HA epitope tag antibody, rabbit anti-hemagglutinin antibody, rabbit anti-HA tag antibody, anti-epitope
Rabbit
Polyclonal
IgG
Target Details
HA-Tag
Conjugated Peptide
Anti-HA antibody was purified from whole rabbit serum prepared by repeated immunizations with the epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH.
This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Application Details
ELISA, IHC, WB
IF, IP, Multiplex
- View References
Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA, immunohistochemistry, and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
Formulation
Liquid (sterile filtered)
1.05 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Vester, SK et al. (2022). SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly. Nature Communications
Applications
WB, IB, PCA
Zajac AL et al. (2022). Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells. Curr Biol.
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Undefined
Fan Y et al. (2022). Cullin 4b Complex Targets IRGM1 to Regulate Intestinal Stem Cell Stemness and Niche. Cell Death Differ.
Applications
WB, IB, PCA
Ulu A et al. (2021). Cdk1 phosphorylation negatively regulates the activity of Net1 towards RhoA during mitosis. Cell Signal.
Applications
IF, Confocal Microscopy
Carr HS et al. (2021). The PDZ domain protein SYNJ2BP regulates GRK-dependent Sst2A phosphorylation and downstream MAPK signaling. Endocrinology.
Applications
IF, Confocal Microscopy
Stevens, LM et al. (2021). Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster. PloS Genetics
Applications
WB, IB, PCA
Landry, NM et al. (2021). SKI activates the Hippo pathway via LIMD1 to inhibit cardiac fibroblast activation. Basic Research in Cardiology
Applications
WB, IB, PCA
Mrowiec K et al. (2021). The periphilin 1-like BFAR isoform 3 is highly expressed in transcriptionally silent oocytes and involved in RNA metabolism. Biochim Biophys Acta Mol Cell Res.
Applications
Undefined
Muroi SI et al. (2021). C-Terminal Domain of Aquaporin-5 Is Required to Pass Its Protein Quality Control and Ensure Its Trafficking to Plasma Membrane. Int J Mol Sci.
Applications
Undefined
Gonclaves-Carneiro D et al. (2021). Origin and evolution of the zinc finger antiviral protein. PLoS Pathog.
Applications
WB, IB, PCA
Hou J et al. (2021). USP18 positively regulates innate antiviral immunity by promoting K63-linked polyubiquitination of MAVS. Nat Commun.
Applications
WB, IB, PCA
Cai B et al. (2021). USP5 attenuates NLRP3 inflammasome activation by promoting autophagic degradation of NLRP3. Autophagy.
Applications
Undefined
May JP. et al. (2020). The Multifunctional Long-Distance Movement Protein of Pea Enation Mosaic Virus 2 Protects Viral and Host Transcripts from Nonsense-Mediated Decay. mBio
Applications
WB, IB, PCA
Woods BL. et al. (2020). Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling. bioRxiv
Applications
WB, IB, PCA
Shi X et al. (2020). Genetically engineered cell-derived nanoparticles for targeted breast cancer immunotherapy. Mol Ther.
Applications
E, EIA
Cousomeau J et al. (2020). Dopamine D2-Like Receptors Modulate Intrinsic Properties and Synaptic Transmission of Parvalbumin Interneurons in the Mouse Primary Motor Cortex. eNeuro.
Applications
IF, Confocal Microscopy
Alvarez-Castelao B et al. (2020). The switch-like expression of heme-regulated kinase 1 mediates neuronal proteostasis following proteasome inhibition. Elife
Applications
IF, Confocal Microscopy
Hoai NB et al. (2020). A Chemogenetic Tool that Enables Functional Neural Circuit Analysis. Cell Rep.
Applications
IHC, ICC, Histology
Pati S et al. (2020). Chronic chemogenetic activation of forebrain excitatory neurons in postnatal life evokes long-lasting changes in mood-related behavior. Elife
Applications
IF, Confocal Microscopy
Riching AS et al. (2020). Suppression of canonical TGF-β signaling enables GATA4 to interact with H3K27me3 demethylase JMJD3 to promote cardiomyogenesis. J Mol Cell Cardiol.
Applications
WB, IB, PCA
Stedden CG et al. (2019). Planar-polarized semaphorin-5c and plexin A promote the collective migration of epithelial cells in Drosophila. Curr Biol.
Applications
IF, Confocal Microscopy
Rohde C et al. (2019). Marburg virus regulates the IRE1/XBP1-dependent unfolded protein response to ensure efficient viral replication. Emerg Microbes Infect.
Applications
WB, IB, PCA
Bohn JA et al. (2019). Flexibility in nucleic acid binding is central to APOBEC3H antiviral activity. J Virol.
Applications
WB, IB, PCA
Nuwer JL et al. (2019). Anterograde trafficking signals in GABAA subunits are required for functional expression. Channels (Austin).
Applications
WB, IB, PCA
Pati S et al. (2019). Chemogenetic activation of excitatory neurons alters hippocampal neurotransmission in a Dose-Dependent manner. eNeuro.
Applications
IF, Confocal Microscopy
Olsen C et al. (2019). Regulation of somatostatin receptor 2 trafficking by C-tail motifs and the retromer. Endocrinology.
Applications
IF, Confocal Microscopy; WB, IB, PCA; Multiplex Assay
Ulu et al. (2018). Stress-activated MAPKs and CRM1 regulate the subcellular localization of Net1A to control cell motility and invasion. Journal of Cell Science
Applications
WB, IB, PCA
Kijima et al. (2018). HSP90 inhibitors disrupt a transient HSP90-HSF1 interaction and identify a noncanonical model of HSP90-mediated HSF1 regulation. Scientific Reports
Applications
WB, IB, PCA
Yue et al. (2018). SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death. Cell Death & Disease
Applications
WB, IB, PCA
Arakaki AKS et al. (2018). The α-arrestin ARRDC3 suppresses breast carcinoma invasion by regulating G protein–coupled receptor lysosomal sorting and signaling. J Biol Chem.
Applications
IF, Confocal Microscopy; WB, IB, PCA
Kostas M et al. (2018). Protein Tyrosine Phosphatase Receptor Type G (PTPRG) Controls Fibroblast Growth Factor Receptor (FGFR) 1 Activity and Influences Sensitivity to FGFR Kinase Inhibitors. Mol Cell Proteomics.
Applications
IP, Co-IP
Yamada T et al. (2018). Mitochondrial stasis reveals p62-mediated ubiquitination in Parkin-independent mitophagy and mitigates nonalcoholic fatty liver disease. Cell Metab.
Applications
IF, Confocal Microscopy; Multiplex Assay
Lv et al. (2017). S. pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity. Molecular Cell
Applications
WB, IB, PCA
Kanter et al. (2017). A Novel Mechanism for the Grid-to-Place Cell Transformation Revealed by Transgenic Depolarization of Medial Entorhinal Cortex Layer II. Neuron
Applications
IHC, ICC, Histology
Blanco-Melo et al. (2017). Co-option of an endogenous retrovirus envelope for host defense in hominid ancestors. Elife
Applications
WB, IB, PCA
Bohn et al. (2017). APOBEC3H structure reveals an unusual mechanism of interaction with duplex RNA. Nature Communications
Applications
WB, IB, PCA
Meese et al. (2017). Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin. Frontiers in Synaptic Neuroscience
Applications
WB, IB, PCA
Yoshii T et al. (2017). Pharmacogenetic reactivation of the original engram evokes an extinguished fear memory. Neuropharmacology.
Applications
IF, Confocal Microscopy; Multiplex Assay
Kim MW et al. (2017). Time-gated detection of protein-protein interactions with transcriptional readout. Elife
Applications
IF, Confocal Microscopy
Wu et al. (2016). Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase. Journal of Biological Chemistry
Applications
WB, IB, PCA
Smith et al. (2016). Cadmium and Secondary Structure-dependent Function of a Degron in the Pca1p Cadmium Exporter. Journal of Biological Chemistry
Applications
WB, IB, PCA
Smith et al. (2016). Endoplasmic Reticulum-associated Degradation of Pca1p, a Polytopic Protein, via Interaction with the Proteasome at the Membrane. Journal of Biological Chemistry
Applications
WB, IB, PCA
York et al. (2016). The RNA Binding Specificity of Human APOBEC3 Proteins Resembles That of HIV-1 Nucleocapsid. PLOS Pathogens
Applications
WB, IB, PCA
Pitoniak et al. (2015). Cdc42p-interacting protein Bem4p regulates the filamentous-growth mitogen-activated protein kinase pathway. Molecular and Cellular Biology
Applications
WB, IB, PCA
Vink et al. (2015). Impact of Adenovirus E4-ORF3 Oligomerization and Protein Localization on Cellular Gene Expression. Viruses
Applications
IF, Confocal Microscopy; WB, IB, PCA
Zhu et al. (2015). Gain-of-function p53 mutants co-opt chromatin pathways to drive cancer growth. Nature
Applications
WB, IB, PCA
Puighermanal E et al. (2015). drd2‐cre:ribotag mouse line unravels the possible diversity of dopamine d2 receptor‐expressing cells of the dorsal mouse hippocampus. Hippocampus.
Applications
IF, Confocal Microscopy; Multiplex Assay
Wei W et al. (2014). YCF1-mediated cadmium resistance in yeast is dependent on copper metabolism and antioxidant enzymes. Antioxid Redox Signal.
Applications
WB, IB, PCA
MacVicar TDB et al. (2014). Impaired OMA1-dependent cleavage of OPA1 and reduced DRP1 fission activity combine to prevent mitophagy in cells that are dependent on oxidative phosphorylation. J Cell Sci.
Applications
WB, IB, PCA
Olmsted ZT et al. (2014). Kinesin-14 and kinesin-5 antagonistically regulate microtubule nucleation by γ-TuRC in yeast and human cells. Nat Commun.
Applications
WB, IB, PCA
Kutluay SB et al. (2014). Global changes in the RNA binding specificity of HIV-1 gag regulate virion genesis. Cell.
Applications
IP, Co-IP; WB, IB, PCA
Oh W et al. (2014). Rho gtpase independent regulation of atm activation and cell survival by the rhogef net1a Cell Cycle
Applications
WB, IB, PCA
Freschi L et al. (2013). qPCA: a scalable assay to measure the perturbation of protein–protein interactions in living cells. Mol Biosyst.
Applications
WB, IB, PCA
Olmsted ZT et al. (2013). Kinesin-14 Pkl1 targets γ-tubulin for release from the γ-tubulin ring complex (γ-TuRC). Cell Cycle.
Applications
WB, IB, PCA
Jiang S et al. (2011). Starch-binding domain-containing protein 1 (Stbd1) and glycogen metabolism: Identification of the Atg8 family interacting motif (AIM) in Stbd1 required for interaction with GABARAPL1. Biochem Biophys Res Commun.
Applications
IF, Confocal Microscopy
Niederkofler V et al. (2010). Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance. Development.
Applications
IHC, ICC, Histology; Multiplex Assay
Chen PY et al. (2009). FRS2 via fibroblast growth factor receptor 1 is required for platelet-derived growth factor receptor beta-mediated regulation of vascular smooth muscle marker gene expression. J Biol Chem.
Applications
IP, Co-IP
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.