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Primary Antibodies  >  Phospho Specific Antibodies
 

Plk1 phospho T210 Antibody

Rabbit Polyclonal
Code

600-401-446
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Affinity Purified Plk1 pT210 was used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.  Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.
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Synonyms: PLK 1 antibody, PLK antibody, polio like kinase antibody, Polo like kinase 1 antibody, Polo-like kinase 1 antibody, Serine/threonine protein kinase 13 antibody

Plk1 phospho T210 Antibody Properties

Description
Anti-Polo-like Kinase (Plk1) pT210 (RABBIT) Antibody - 600-401-446
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:3,000 - 1:12,000
Western Blot : 1:200 - 1:2,000
Immunohistochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PLK1
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Lot#  

Plk1 phospho T210 Antibody Description

Background
Polo-like Kinase pT210 Antibody detects phosphorylated Plk1 protein. Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G1 or S phase and is associated with the phosphorylation of cyclin B1.  Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression.  Plk-1 has a nuclear localization and accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.  Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.
Immunogen
Anti-Polo-like Kinase pT210 Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to aa 205-214 of Human Polo-like kinase 1 (Plk1) protein.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity-purified antibody has been tested for use in ELISA, IHC and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 68 kDa in size corresponding to Plk-1 by western blotting in the appropriate cell lysate or extract.   This antibody is positive by IHC on kidney, liver, cancer, thyroid and lymphocyte tissue.
Purity/Specifity
Anti-Polo-like Kinase pT210 Antibody is directed against human phosphorylated Plk1 protein. This antibody was affinity purified. This antibody is specific for phosphorylated human Plk-1 protein at the pT210 residue.  BLAST analysis indicates 100 % homology of the immunizing sequence with Plk-1 homologues from human, chimpanzee, pig, chicken, mouse, rat, Xenopus, dog, mosquito, zebra fish, starfish, sea urchin and fruit fly.  Cross reactivity with Plk-1 protein homologues from C.elgans and honeybee may also occur as sequence homology varies by one amino acid residue in this sequence.  Reactivity with Plk-1 protein from other sources is not known.  Minimal reactivity is expected with the non-phosphorylated form of the protein.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
van Vugt,M.A., Br and Medema,R.H. (2004) Polo-like kinase-1 controls recovery from a G2 DNA damage-induced arrest in mammalian cells. Mol. Cell 15 (5), 799-811. van Vugt,M.A. et al. (2004) Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/Cdc20 activation and initiation of cytokinesis. J. Biol. Chem. 279 (35), 36841-36854. Weichert,W., Schmidt,M., Gekeler,V., Denkert,C., Stephan,C., Jung,K., Loening,S., Dietel,M. and Kristiansen,G. (2004) Polo-like kinase 1 is overexpressed in prostate cancer and linked to higher tumor grades. Prostate 60 (3), 240-245. Gunawardena,R.W., Siddiqui,H., Solomon,D.A., Mayhew,C.N., Held,J., Angus,S.P. and Knudsen,E.S. (2004) Hierarchical requirement of SWI/SNF in retinoblastoma tumor suppressor-mediated repression of Plk1. J. Biol. Chem. 279 (28), 29278-29285. Ando,K., Ozaki,T., Yamamoto,H., Furuya,K., Hosoda,M., Hayashi,S., Fukuzawa,M. and Nakagawara,A. (2004) Polo-like kinase 1 (Plk1) inhibits p53 function by physical interaction and phosphorylation. J. Biol. Chem. 279 (24), 25549-25561. Litvak,V., Argov,R., Dahan,N., Ramachandran,S., Amarilio,R., Shainskaya,A. and Lev,S. (2004) Mitotic phosphorylation of the peripheral Golgi protein Nir2 by Cdk1 provides a docking mechanism for Plk1 and affects cytokinesis completion. Mol. Cell 14 (3), 319-330.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-160

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-A17

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, mouse, rat
Catalog Number
200-301-160

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
600-401-465

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry