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Primary Antibodies > DNA Damage and Repair Antibodies

BACH1 Antibody

Rabbit Polyclonal

Code

600-401-458

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's Affinity Purified anti-BACH1 antibody shows detection of a band at ~105 kDa (lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (arrowhead). Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction. Approximately 35 ?g of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

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Synonyms: BRAC 1 Associated C Terminal Helicase 1 antibody, BRCA 1 Interacting Protein 1 antibody, BRCA1 binding helicase like protein BACH1 antibody, BRCA1 interacting protein C terminal helicase 1 antibody, BRIP 1 antibody

Datasheet
References
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BACH1 Antibody Properties

Description

Anti-Human BACH1 (RABBIT) Antibody - 600-401-458

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:10,000 - 1:44,000
Western Blot : 1:500- 1:2,000
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

BACH1

BACH1 Antibody Description

Background

BACH1 (also known as BRCA1 interacting protein C-terminal helicase 1, BRCA1-interacting protein 1 and BRCA1-associated C-terminal helicase 1) is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). The BACH1 gene may be a target of germline cancer-inducing mutations. BACH1 is localized within the nucleus and functions as a DNA-dependent ATPase and 5' to 3' DNA helicase. Two isoforms have been identified for this protein.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 92-104 of isoform 1 of human BACH1 protein.

Immunogen Type

Recombinant Protein

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 105 -140 kDa in size corresponding to isoforms of BACH1 protein by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This affinity purified antibody is directed against human BACH1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with BACH1 protein from human (100% homology) and chimpanzee (92% homology). Expect reactivity with isoform 1 and isoform 2 of BACH1. Reactivity against BACH1 homologues from rat and mouse is not expected. Reactivity against homologues from other sources is not known.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Gupta,R., Sharma,S., Sommers,J.A., Jin,Z., Cantor,S.B. and Brosh,R.M. Jr. (2005) Analysis of the DNA substrate specificity of the human BACH1 helicase associated with breast cancer. J. Biol. Chem. 280 (27), 25450-25460. Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E., Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P. (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 101 (33), 12130-12135. Clapperton,J.A., Manke,I.A., Lowery,D.M., Ho,T., Haire,L.F., Yaffe,M.B. and Smerdon,S.J. (2004) Structure and mechanism of BRCA1 BRCT domain recognition of phosphorylated BACH1 with implications for cancer. Mol. Cell 11 (6), 512-518. Ohira,M., Seki,N., Nagase,T., Ishikawa,K., Nomura,N. and Ohara,O. (1998) Characterization of a human homolog (BACH1) of the mouse Bach1 gene encoding a BTB-basic leucine zipper transcription factor and its mapping to chromosome 21q22.1. Genomics 47 (2), 300-306. Oyake,T., Itoh,K., Motohashi,H., Hayashi,N., Hoshino,H., Nishizawa,M., Yamamoto,M. and Igarashi,K. (1996) Bach proteins belong to a novel family of BTB-basic leucine zipper transcription factors that interact with MafK and regulate transcription through the NF-E2 site. Mol. Cell. Biol. 16 (11), 6083-6095.

Specific Reference

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