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Primary Antibodies  >  DNA Damage and Repair Antibodies
 

Rad9 S1260 Antibody

Rabbit Polyclonal
Code

600-401-467
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Synonyms: Cell cycle checkpoint control protein antibody, Cell cycle checkpoint control protein RAD9A antibody, DNA repair exonuclease rad9 homolog A antibody

Rad9 S1260 Antibody Properties

Description
Anti-Yeast Rad9 S1260 (pan reactive) (RABBIT) Antibody - 600-401-467
Host
Rabbit
Target Species
Yeast
Known Cross Reactivity
Saccharomyces cerevisiae
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:500- 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.1 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
RAD9
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Lot#  

Rad9 S1260 Antibody Description

Background
Cells respond to DNA damage by activating a network of signaling pathways that control cell cycle progression and DNA repair.  Cell cycle checkpoints are mechanisms that transiently delay cell cycle progression when DNA is damaged or DNA replication is incomplete. In the fission yeast Schizosaccharomyces pombe, a group of six such checkpoint control genes have been identified and include rad1+, rad3+, rad9+, rad17+, rad26+ and hus1+. Mutations in any one of these genes render cells sensitive to gamma-rays, UV light or the DNA synthesis inhibitor hydroxyurea (HU) and eliminate the ability of cells to delay entry into mitosis after treatment with these agents. All of these genes apparently link abnormal DNA structures to cell cycle control.  As for cell cycle-related genes in general, these checkpoint control genes are highly conserved throughout evolution. Human and mouse versions of several of the S.pombe genes have been isolated, providing strong evidence that checkpoint control mechanisms are also highly conserved. In mammals, these genes are thought to maintain genomic stability, especially in the presence of DNA damage. Therefore, when these genes are altered, genomic instability may occur and lead to cancer.  The biochemical activities of most of the checkpoint control gene products are not well established, although progress has been made towards learning more about their function. For example, examination of the structure of the protein encoded by human or S.pombe rad9 reveals a BH3-like domain in the N-terminal region that can bind the anti-apoptotic proteins Bcl-2 and Bcl-XL. Furthermore, overexpression of the gene from either organism in human cells can cause apoptosis in a BH3 domain-dependent manner. Both S.pombe and human versions of the protein can bind two other checkpoint control proteins, Hus1p (HUS1p) and Rad1p (RAD1p). Human RAD9 protein binds HUS1 and RAD1 proteins at its C-terminal region, suggesting that RAD9 has at least two functional domains, one involved in apoptosis and the other in cell cycle checkpoint control.  Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p; is hyperphosphorylated by Mec1p and Tel1p; and is a potential Cdc28p substrate.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 1249-1263 of 1309 of yeast RAD9 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA against the immunizing peptide.  Reactivity in other immunoassays is unknown. Mature RAD9 is reported to have an apparent molecular weight of ~140kDa.
Purity/Specifity
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.  No reactivity is expected against the human analog of RAD9.  Reactivity against RAD9 from other sources is unknown.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
H.Hang, S.J. Rauth, K.M. Hopkins and H.B. Lieberman (2000) Mutant alleles of Schizosaccharomyces pombe rad9+ alter hydroxyurea resistance, radioresistance and checkpoint control.  Nucleic Acids Research 28:21; 4340-4349. Aboussekhra, A. , Vialard, J.E. , Morrison, D.E. , de la Torre-Ruiz, M.A. , Cernakova, L. , Fabre, F. and Lowndes, N.F. (1996) A novel role for the budding yeast RAD9 checkpoint gene in DNA damage-dependent transcription. EMBO J., 15, 3912-3922 Burtelow, M.A., Roos-Mattjus, P.M., Rauen, M., Babendure, J.R., and Karnitz, L.M. (2001). Reconstitution and molecular analysis of the hRad9-hHus1-hRad1 (9-1-1) DNA damage-responsive checkpoint complex. J. Biol. Chem. 276, 25903-25909 Cai, R.L., Yan-Neale, Y., Cueto, M.A., Xu, H., and Cohen, D. (2000). HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins. J. Biol. Chem. 275, 27909-27916 Lieberman, H.B., Hopkins, K.M., Nass, M., Demetrick, D., and Davey, S. (1996). A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene. Proc. Natl. Acad. Sci. USA 93, 13890-13895
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Saccharomyces cerevisiae
Catalog Number
600-401-346

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Saccharomyces cerevisiae
Catalog Number
600-401-468

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry