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Primary Antibodies  >  Transcription Factor Antibodies
 

EGR-1 Antibody

Rabbit Polyclonal
Code

600-401-693
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Rockland's Affinity Purified anti-EGR-1 antibody was used at a 10 µg/ml to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded.
Rockland's Affinity Purified anti-EGR-1 antibody was used at a 10 µg/ml to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded.
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Shipping:
$85.00 to United States
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$20.00
Synonyms: AT225 antibody, Early growth response 1 antibody, G0S30 antibody, Krox 24 protein antibody, KROX24 antibody, Nerve growth factor-induced protein A antibody

EGR-1 Antibody Properties

Description
Anti-EGR-1 (RABBIT) Antibody - 600-401-693
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, chimpanzee, mouse
Clonality
Polyclonal
Application
ELISA : 1:4,000 - 1:16,000
Western Blot : 1:500 - 1:3,000
Immunochemistry: 2 µg/ml to 20 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.93 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
EGR-1
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Lot#  

EGR-1 Antibody Description

Background
EGR-1 (also called Early Growth Response protein 1, Krox-24 protein, ZIF268, Nerve growth factor-induced protein A or NGFI-A, Transcription factor ETR103, and Zinc finger protein 225 or AT225) is a transcriptional regulator that recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site).  EGR-1 activates the transcription of target genes whose products are required for mitogenesis and differentiation.   EGR-1 is a nuclear protein induced by growth factors. Expression has been identified in a variety of cancers.
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 94-108 (eqpyehltaesfpdi) of Human EGR-1.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~58 kDa in size corresponding to EGR-1 by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity purified antibody is directed against human EGR-1 protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with EGR-1
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Suggs,S.V., Katzowitz,J.L., Tsai-Morris,C. and Sukhatme,V.P. (1990) cDNA sequence of the human cellular early growth response gene Egr-1. Nucleic Acids Res. 18 (14), 4283. Shimizu,N., Ohta,M., Fujiwara,C., Sagara,J., Mochizuki,N., Oda,T. and Utiyama,H. (1992) A gene coding for a zinc finger protein is induced during 12-O-tetradecanoylphorbol-13-acetate-stimulated HL-60 cell differentiation. J. Biochem. 111 (2), 272-277. Wright,J.J., Gunter,K.C., Mitsuya,H., Irving,S.G., Kelly,K. and Siebenlist,U. (1990) Expression of a zinc finger gene in HTLV-I- and HTLV-II-transformed cells. Science 248 (4955), 588-591.
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Kits;
Applications
ELISA, WB, IHC, IF, IP
Catalog Number
K-500

Product Type
Supporting Reagents;
Applications
ELISA, WB, IHC, IF
Catalog Number
K505

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-0100

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry