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Primary Antibodies  >  Neuroscience Antibodies
 

Gli-3 Antibody

Rabbit Polyclonal
Code

600-401-694
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Immunohistochemistry of Rabbit anti-Gli-3 antibody.  
This image tissue:  human glioblastoma.  
Specific staining was also noted in tissue from adrenal, brain, glioblastoma, colon, heart, kidney, lung, liver, skeletal muscle, ovary, pancreas, placenta, skin, spleen, stomach, testes, thymus, thyroid, tonsil and uterus.
Fixation:  formalin fixed paraffin embedded.  
Antigen retrieval:  not required.
Primary antibody:  Gli-3 antibody at  0.625 µg/ml for 1 h at RT.
Secondary antibody:  Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.
Localization:  Gli-3 is nuclear and smooth muscle.
Staining:  Gli-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of Rabbit anti-Gli-3 antibody.  
Lane 1:  50 kDa molecular weight marker.  
Lane 2:  293T cells transfected with CrkL-Flag.  Lane 3:  293T cells transfected with human Gli-3.  
Load:  35 µg per lane.
Primary antibody:  Gli-3 antibody at 1:400 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  170-190 kDa for hGli-3. 
Other band(s): Non specific background ~60kDa.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Gli-3, Zinc Finger Protein Gli-3, GLI-Kruppel family member GLI-3

Gli-3 Antibody Properties

Description
Anti-Gli-3 (RABBIT) Antibody - 600-401-694
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail
Clonality
Polyclonal
Application
ELISA : 1:6,000 - 1:30,000
Western Blot : 1:500 - 1:2,000
Immunochemistry: 0.5 mg/ml - 5 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
GLI3
Download CofA
Lot#  

Gli-3 Antibody Description

Background
Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and cranio-facial development.  Two isoforms of human Gli-3 have been reported.  One is the full-length protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.
Immunogen
This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to amino acids 41-57 of human Gli-3 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.
Purity/Specifity
This affinity-purified antibody is directed against human Gli-3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.   A BLAST analysis was used to suggest cross reactivity with Gli-3 from human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail based on 100% sequence homology with the immunogen. Reactivity is also expected against homologues from mouse (94%) and rat (88%) based on partial homology.  Reactivity with Gli-3 from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Johnston,J.J., et al. (2005) Molecular and clinical analyses of Greig cephalopolysyndactyly and Pallister-Hall syndromes: robust phenotype prediction from the type and position of GLI3 mutations. Am. J. Hum. Genet. 76 (4), 609-622. Mendoza-Londono,R., Kashork,C.D., Shaffer,L.G., Krance,R. and Plon,S.E. (2005) Acute lymphoblastic leukemia in a patient with Greig cephalopolysyndactyly and interstitial deletion of chromosome 7 del(7)(p11.2 p14) involving the GLI3 and ZNFN1A1 genes. Genes Chromosomes Cancer 42 (1), 82-86. Johnston,J.J., Olivos-Glander,I., Turner,J., Aleck,K., Bird,L.M., Mehta,L., Schimke,R.N., Heilstedt,H., Spence,J.E., Blancato,J. and Biesecker,L.G. (2003) Clinical and molecular delineation of the Greig cephalopolysyndactyly contiguous gene deletion syndrome and its distinction from acrocallosal syndrome. Am. J. Med. Genet. 123 (3), 236-242.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mouse, human
Catalog Number
100-401-223

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mouse
Catalog Number
600-401-695

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
600-401-905

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-928

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry