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Primary Antibodies  >  Neuroscience Antibodies
 

Gli-2 Antibody

Rabbit Polyclonal
Code

600-401-695
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Rockland's Affinity Purified anti-mouse Gli-2 antibody was used at 10 µg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues, with low background staining.  This image shows Gli-2 staining of mouse testis. Tissue was formalin-fixed and paraffin embedded.
Western blot using Rockland's Affinity Purified anti-Gli-2 antibody shows detection of a predominant band at ~190 kDa corresponding to Gli-2 (arrowhead) in mouse brain whole cell lysate (lane 1).  Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (lane 2). ~ 25 µg of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.   After blocking with 5% goat  serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:750.  Incubation was at room temperature for 2 h followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (611-132-122) for 45 min at room temperature.  Molecular weight markers are shown (M) using the 700 nm channel (red).  IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.
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Synonyms: Gli 2 antibody, GLI family zinc finger 2 antibody, GLI kruppel family member 2 antibody, GLI Kruppel family member GLI2 antibody, Oncogene GLI2 antibody, Tax helper protein antibody, Tax helper protein 2 antibody

Gli-2 Antibody Properties

Description
Anti-Gli-2 (RABBIT) Antibody - 600-401-695
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse
Clonality
Polyclonal
Application
ELISA : 1:15,000 - 1:60,000
Western Blot : 1:500 - 1:2,000
Immunochemistry: 2 µg/ml to 20 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.02 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
GLI2
Download CofA
Lot#  

Gli-2 Antibody Description

Background
Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling, and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes: Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, pre-axial
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by WB and IHC.  Multiple splice variants have been reported for this protein.
Purity/Specifity
This affinity purified antibody is directed against mouse Gli-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross-reactivity with Gli-2 from mouse and rat sources based on 100% sequence homology with the immunogen. Reactivity with Gli-2 from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Kim JH, Huang Z, Mo R (2005) Gli3 null mice display glandular overgrowth of the developing stomach. Dev. Dyn. 234(4):984-91. McDermott A, Gustafsson M, Elsam T, Hui CC, Emerson CP Jr, Borycki AG (2005) Gli2 and Gli3 have redundant and context-dependent function in skeletal muscle formation. Development 132(2):345-57. Dillon AK, Fujita SC, Matise MP, Jarjour AA, Kennedy TE, Kollmus H, Arnold HH, Weiner JA, Sanes JR, Kaprielian Z (2005) Molecular control of spinal accessory motor neuron/axon development in the mouse spinal cord. J. Neurosci. 25(44):10119-30.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mouse, human
Catalog Number
100-401-223

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail
Catalog Number
600-401-694

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
600-401-905

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-928

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry