GLI2 Antibody
Anti-Gli-2 Antibody - Western Blot
Immunohistochemistry - Gli-2 Antibody
Western Blot of Anti-GLI2
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Datasheet

GLI2 Antibody

Rabbit Polyclonal

4 References
600-401-845S 600-401-845
25 µL 100 µg
WB, ELISA, IHC
Human, Rat
Rabbit
$125.00 /Per Item
$475.00 /Per Item
25 µL $125.00
100 µg $475.00
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Availability: Call for availability Availability: Ships on approximately 09/02/2024
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Background

Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila.

Product Details

Anti-GLI2 (RABBIT) Antibody - 600-401-845
rabbit anti-GLI-2 antibody, GLI2, zinc finger protein GLI2, GLI family zinc finger protein 2, Tax helper protein antibody, THP antibody
Rabbit
Polyclonal
IgG

Target Details

GLI2  - View All GLI2 Products
Human, Rat
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human Gli-2 (isoform a).
This affinity-purified antibody is directed against human Gli-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.   A BLAST analysis was used to suggest cross reactivity with Gli-2 from human and chimpanzee based on the immunizing sequence.
NCBI - NP_005261.2
UniProtKB - P10070
NCBI - NP_084655
GeneID - 2736

Application Details

ELISA, IHC, WB
This antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by western blot.  Multiple splice variants have been reported for this protein a, b, g and d (133.3, 131.6, 88.1 and 86.4 kDa respectively).  Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Formulation

Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Patched 1 Expression Correlates with Biochemical Relapse in High-Risk Prostate Cancer Patients. The American Journal of Pathology
Applications
IHC, ICC, Histology
(). The Effect of Metformin and GANT61 Combinations on the Radiosensitivity of Prostate Cancer Cells. International Journal of Molecular Sciences
Applications
WB, IB, PCA
(). Tissue microarray analysis indicates hedgehog signaling as a potential prognostic factor in intermediate-risk prostate cancer. BMC Cancer
Applications
IHC, ICC, Histology
(). The hedgehog inhibitor GANT61 sensitizes prostate cancer cells to ionizing radiation both in vitro and in vivo. Oncotarget
Applications
WB, IB, PCA

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If you need help finding your CoA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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