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Primary Antibodies  >  Neuroscience Antibodies
 

CaM Kinase II Antibody

Rabbit Polyclonal
Code

600-401-873
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's Affinity Purified anti-CaM Kinase II antibody shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II (arrowhead lane 1).  Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2).  Approximately 35 ?g of a mouse brain whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.  CaM Kinase II was similarly detected on lysates from rat brain (not shown).  After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.
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Synonyms: Calcium/calmodulin dependent protein kinase II alpha antibody, CaM kinase II alpha antibody, CaM kinase II alpha chain antibody, CaM kinase II alpha subunit antibody, CaM kinase II antibody, CaM kinase II beta antibody

CaM Kinase II Antibody Properties

Description
Anti-CaM Kinase II (RABBIT) Antibody - 600-401-873
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat, dog, orangutan
Clonality
Polyclonal
Application
ELISA : 1:4,000 - 1:20,000
Western Blot : 1:1,000 - 1:3,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.57 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CAMK2A
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Lot#  

CaM Kinase II Antibody Description

Background
CaM Kinase II (also known as CAMK2 and calcium/calmodulin-dependent protein kinase type II alpha chain) is a prominent kinase in the central nervous system that may function in the long-term potentiation of neurotransmitter release.  CaM Kinase II autophosphorylates itself at Thr-286 which allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state. CaM Kinase II is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. This kinase is expressed in brain tissue.  Alternative splicing occurs for this gene product.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 6-23 of Human CaM Kinase II protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 54 kDa in size corresponding to CaM Kinase II by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity purified antibody is directed against human CaM Kinase II protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat, dog and orangutan based on 100% homology for the immunogen sequence. Cross reactivity with CaM Kinase II protein from zebrafish, chiken, pig, rabbit and frog may occur as this sequence only varies by one amino acid residue (94% homology). Cross reactivity with CaM Kinase II homologues from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Lin,C.R., Kapiloff,M.S., Durgerian,S., Tatemoto,K., Russo,A.F., Hanson,P., Schulman,H. and Rosenfeld,M.G. (1987) Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase. Proc. Natl. Acad. Sci. U.S.A. 84 (16), 5962-5966. Bulleit,R.F., Bennett,M.K., Molloy,S.S., Hurley,J.B. and Kennedy,M.B. (1988) Conserved and variable regions in the subunits of brain type II Ca2+/calmodulin-dependent protein kinase. Neuron 1 (1), 63-72. Hanley,R.M., Means,A.R., Ono,T., Kemp,B.E., Burgin,K.E., Waxham,N. and Kelly,P.T. (1987) Functional analysis of a complementary DNA for the 50-kilodalton subunit of calmodulin kinase II. Science 237 (4812), 293-297.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat, dog, bovine, chimpanzee
Catalog Number
100-401-883

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Kits;
Reacts With
n/a
Catalog Number
KCB003

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry