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Primary Antibodies  >  Cancer Research Antibodies
 

BORIS Antibody

Rabbit Polyclonal
Code

600-401-907
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's Affinity Purified anti-BORIS antibody shows detection of a predominant band corresponding to BORIS in human tissue lysates (arrowhead).   Lane 1 contains lysate from human prostate tissue.  Lane 2 contains lysate from human spleen tissue.  A predominant band at ~75 kDa is observed.  Molecular weight estimation was made by comparison to prestained MW markers as indicated.
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Synonyms: Brother of the regulator of imprinted sites antibody, CCCTC binding factor (zinc finger protein) like antibody, CCCTC-binding factor antibody, CTCF paralog antibody, CTCF T antibody

BORIS Antibody Properties

Description
Anti-BORIS (RABBIT) Antibody - 600-401-907
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, chimpanzee
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:200 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.28 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CTCFL
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Lot#  

BORIS Antibody Description

Background
Anti-BORIS antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. BORIS (Brother of the Regulator of Imprinted Sites) also known as  CCCTC-binding factor-like protein, is  normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development.  However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers.  Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation.  BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line.  BORIS is found in both the nucleus and cytoplasm. BORIS antibody can be used to investigate epigenetic regulation.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 9-26 of human BORIS protein.
Immunogen Type
Peptide
Storage Condition
Store BORIS Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
BORIS affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 75 kDa in size corresponding to BORIS by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity-purified antibody is directed against the human BORIS protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross reactivity with BORIS proteins from human and chimpanzee.  Partial reactivity may occur against BORIS from mouse, dog and rat sources based on varying degrees of homology to the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Loukinov D.I., et al. (2002) BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma. Proc. Natl. Acad. Sci. U.S.A. 99:6806-6811. Shaw P.H., Jelinic P. (2001) Human CTCF homolog, expressed uniquely in testis. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases. Klenova E.M., Morse H.C. III, Ohlsson R., Lobanenkov V.V.; (2002) The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer. Semin. Cancer Biol. 12:399-414.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-224

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
600-401-381

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry