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Primary Antibodies  >  Cancer Research Antibodies
 

SPANX Antibody

Rabbit Polyclonal
Code

600-401-916
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17 kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead).  VWM105 cells are derived from a human melanoma and are positive for SPANX proteins.  Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein.  The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins.  Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000.  IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) was used (left).  IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated.  Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
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Synonyms: Cancer-testis-associated protein CTp11 antibody, CTP11 antibody, Nuclear associated protein SPAN Xc antibody, SPANX family, member C antibody

SPANX Antibody Properties

Description
Anti-SPANX (pan) (RABBIT) Antibody - 600-401-916
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:500 - 1:3,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SPANXC
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Lot#  

SPANX Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Human Sperm Proteins Associated with the Nucleus on X-chromosome (SPANX) are relatively low molecular weight cytoplasmic proteins found in testis and sperm.  Their expression in other tissues indicates malignancies. Family members are observed as proteins that range from 15 to 20 kDa.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 31-47 of human SPANX-C protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a
Purity/Specifity
This affinity-purified antibody is directed against human SPANX proteins.  The immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.   A BLAST analysis was used to suggest cross reactivity with SPANX proteins from gorilla and chimpanzee.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Kouprina N, Mullokandov M, Rogozin I, Collins K, Solomon G, Risinger J,Koonin E, Barrett JC. Larionov V. (2004) The SPANX gene family of cancer-testis specific antigens: rapid evolution, an unusual case of positive selection and amplification in African great apes and hominids. Proc Natl Acad Sci USA 101: 3077-3082. Wang Z, Zhang Y, Liu H, Salati E, Chiriva-Internati M, Lim SH. (2003) Gene expression and immunologic consequence of SPAN-Xb in myeloma and other hematologic malignancies. Blood 101: 955-960. Westbrook VA, Diekman AB, Klotz KL, Khole VV, von Kap-Herr C, Golden WL, Eddy RL, Shows TB, Stoler MH, Lee CY, Flickinger CJ, Herr JC. (2000)Spermatid-specific expression of the novel X-linked gene product SPAN-X localized to the nucleus of human spermatozoa. Biol Reprod 63: 469-481.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
200-401-915

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-401-C43

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-401-C43S

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, chimpanzee, macaque
Catalog Number
600-401-A46

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry