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Primary Antibodies  >  Phospho Specific Antibodies
 

Mlf1 phospho T78 Antibody

Rabbit Polyclonal
Code

600-401-930
Size

100 µg
Price

$309.00
Availability

Ships within 2 weeks.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Western blot using Rockland's affinity purified anti-MLF1IP pT78 antibody shows detection of MLF1IP phosphorylated at Thr78. HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1. Blots were probed with the anti-MLF1IP pT78 antibody, stripped, and then reprobed with anti-GFP antibody (Kang & Park, et al., 2006).
Rockland's affinity purified anti-MLF1IP pT78 antibody was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X.  Tissue was formalin-fixed and paraffin embedded.  The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.  Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.
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Synonyms: FLJ23468 antibody, HHV8 LNAIP1 antibody, ICEN24 antibody, Kaposi Sarcoma Herpesvirus latent nuclear antigen interacting protein 1 antibody

Mlf1 phospho T78 Antibody Properties

Description
Anti-MLF1 Interacting Protein pT78 (RABBIT) Antibody - 600-401-930
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, dog, bovine, chimpanzee
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:2,000
Immunochemistry: 20 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.06 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
MLF1IP
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Lot#  

Mlf1 phospho T78 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate.  Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/ anaphase transition.  Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry.  Mutations of these sites stabilize MLF1IP and inhibit mitotic progression.  Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation.  Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block.  Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset.  MLF1IP is speculated to be a novel tumor suppressor,
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein.  The immunogen peptide is phosphorylated at Thr78.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, western blotting and immunocytochemistry.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 65 kDa in size corresponding to MLF1IP protein by western blotting in the appropriately stimulated tissue, cell lysate or extract.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against the non-phospho peptide.  This antibody is specific for human MLF1IP protein phosphorylated at Thr78.  A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, dog, bovine and chimpanzee based on 100% homology with the immunizing sequence.  Expect partial reactivity with homologues from rat and mouse (90% homology).   Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Hanissian,S.H., Teng,B., Akbar,U., Janjetovic,Z., Zhou,Q., Duntsch,C. and Robertson,J.H. (2005) Regulation of myeloid leukemia factor-1 interacting protein (MLF1IP) expression in glioblastoma. Brain Res. 1047 (1), 56-64. Hanissian,S.H., Akbar,U., Teng,B., Janjetovic,Z., Hoffmann,A., Hitzler,J.K., Iscove,N., Hamre,K., Du,X., Tong,Y., Mukatira,S., Robertson,J.H. and Morris,S.W. (2004) cDNA cloning and characterization of a novel gene encoding the MLF1-interacting protein MLF1IP. Oncogene 23 (20), 3700-3707.
Specific Reference
Kang, Y.H., Park, J.E., et al. (2006) Self-Regulated Plk1 Recruitment to Kinetochores by the Plk1-PBIP1 Interaction Is Critical for Proper Chromosome Segregation. Mol. Cell 24, 409-422.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-A91

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-A91S

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry