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Lysine Acetylated Antibody

Rabbit Polyclonal
NCI Collaboration
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Rockland's Affinity Purified anti-Acetylated Lysine (AcK) antibody is shown to detect acetylated histone in TSA-treated mouse spleen cell lysate (Panel A); control (left lane) and TSA-treated mouse spleen cell lysate (right lane) in panel B; and in acetylated BSA loaded as indicated (panel C).
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Number:
600-401-939
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Lysine Acetylated Antibody Properties

Description
Anti-Lysine Acetylated (AcK) (RABBIT) Antibody - 600-401-939
Host
Rabbit
Known Cross Reactivity
mammalian, avian
Clonality
Polyclonal
Application
ELISA : 1:1,000-1:2,500
IF Microscopy : User Optimized
Flow Cytometry : User Optimized
Western Blot : 1:500-1:1,000
Immunohistochemistry: User Optimized
ImmunoPrecipitation: 10 µg/mg protein sample
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
None
Stabilizer
50% (v/v) Glycerol
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Lysine Acetylated Antibody Description

Background
Acetylation refers to the process of introducing an acetyl group into a compound. Proteins are typically acetylated on lysine residues and this reaction relies on acetyl-coenzyme A as the acetyl group donor. Acetylated Lysine antibody is useful in Cancer, Cell Biology and Chromatin & Nuclear Signaling research.
Synonyms
acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody
Immunogen
Anti-Lysine Acetylated Antibody was prepared from whole rabbit serum produced by repeated immunizations with acetylated lysine conjugated KLH.
Immunogen Type
Other
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Lysine Acetylated Antibody is suitable for use in ELISA, western blotting, immunofluorescence microscopy, and immunoprecipitation assays. Although not tested, this antibody is likely functional in RIA, flow cytometry, and immunohistochemistry.
Purity/Specifity
Anti-Lysine Acetylated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using acetylated lysine peptide coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.  The antibody reacts specifically with acetylated lysine residues.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mammalian and avian
Catalog Number
200-301-A97

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Secondary Antibodies;
Applications
FLISA, WB, IF
Reacts With
Rabbit
Catalog Number
611-143-002

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry