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Primary Antibodies  >  Chromatin Antibodies
 

PCNA Antibody

Rabbit Polyclonal
Code

600-401-968
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's affinity purified anti-PCNA antibody shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts.  Approximately 25 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose.  After blocking, the membrane was incubated with the primary antibody diluted to 1:1000.  The membrane was washed and reacted with a 1:10,000 dilution of IRDye® 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 (611-132-122) (800 nm channel, green).  Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red).  Other detection systems will yield similar results.
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Synonyms: PCNA, proliferating cell nuclear antigen, cyclin

PCNA Antibody Properties

Description
Anti-PCNA (RABBIT) Antibody - 600-401-968
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, monkey, dog, mouse, rat, bovine, chicken, fish, Xenopus
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:8,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.69 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PCNA
Download CofA
Lot#  

PCNA Antibody Description

Background
The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the processibility of DNA polymerase during elongation of the leading strand.  PCNA is expressed in the nucleus of all proliferating cells and is pivotal for DNA synthesis and cell cycle progression. In response to DNA damage, PCNA is mono-ubiquitinated and is involved in mismatch-provoked excision.  PCNA is a useful marker for DNA synthesis and is highly conserved among most species.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PCNA protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29-36 kDa in size corresponding to PCNA protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity purified antibody is directed against human PCNA protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross reactivity with PCNA protein from human, monkey, dog, mouse, rat, bovine, chicken, fish and Xenopus based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Chuang, L.S.-H., Ian, H.-I., Koh, T.-W., Ng, H.-H., Xu, G., Li, B.F.L. (1997) Human DNA-(cytosine-5) methyltransferase-PCNA complex as a target for p21WAF1. Science 277:1996-2000. Dzantiev, L., Constantin, N., Genschel, J., Iyer, R.R., Burgers, P.M., Modrich, P. (2004) A defined human system that supports bidirectional mismatch-provoked excision. Mol. Cell 15:31-41. Haracska, L., Unk I., Johnson, R.E., Phillips, B.B., Hurwitz, J., Prakash, L., Prakash, S. (2002) Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA. Mol. Cell. Biol. 22:784-791.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-400

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Human
Catalog Number
200-C01-B34

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
Algal
Catalog Number
600-401-887

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry