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Glutathione Peroxidase 4 Antibody

Rabbit Polyclonal
NCI Collaboration
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Western blot using Rockland's affinity purified anti-GPx4 to detect GPx4 in testis extract (arrow). Tissue extract (40 µg) was electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. Personal Communication, Dolph Hatfield, CCR-NCI, Bethesda, MD.
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Number:
600-401-972
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Glutathione Peroxidase 4 Antibody Properties

Description
Anti-Glutathione Peroxidase 4 (Gpx4) (RABBIT) Antibody - 600-401-972
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse, rat, guinea pig
Clonality
Polyclonal
Application
ELISA : 1:7,000 - 1:30,000
Western Blot : 1:500 - 1:3,000
Immunohistochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.15 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
GPX4
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Glutathione Peroxidase 4 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Glutathione Peroxidase 4 (GPx4, also known as PHGPx) functions as an antioxidant and also plays a role in redox regulation, sexual maturation, inflammation, apoptosis, and differentiation.  GPx4 was found to have an important role in preventing lipid peroxidation, and has been discussed primarily as the glutathione peroxidase protecting biomembranes against oxidative stress.  GPx4 can react with free hydrogen peroxide as well as with a wide range of lipid hydroperoxides, including those derived from cholesterol and cholesteryl esters.  GPx4 is the only GPx to use phospholipid hydroperoxides as substrates.  It can also reduce thymine hydroperoxide. In contrast to GPx1 and GPx3, GPx4 can use a wide range of reducing substrates in addition to glutathione.  GPx4 is expressed in high levels in testes, suggesting a role in male fertility.  It has also been identified as an enzymatically inactive structural protein of the mitochondrial capsule of sperm.
Synonyms
GPX4 antibody, MCSP antibody, PHGPx antibody, Phospholipid hydroperoxidase antibody, Phospholipid hydroperoxide glutathione peroxidase antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse GPx4 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 19 kDa in size corresponding to GPx4 protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity purified antibody is directed against mouse GPx4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross-reactivity with GPx4 protein from mouse, rat and guinea pig based on 100% homology with the immunizing sequence.  Cross-reactivity with human GPx4 is also predicted based on a 94% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-321

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, primate
Catalog Number
109-401-306

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
600-401-986

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry