14-12_peptide top banner
 
Primary Antibodies  >  Cancer Research Antibodies
 

Rab11 FIB3 Antibody

Rabbit Polyclonal
NCI Collaboration
Order Offline
Number:
600-401-994
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Rab11 FIB3 Antibody Properties

Description
Anti-Rab11 Family-Interacting Protein 3 (FIP3) (RABBIT) Antibody - 600-401-994
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:200 - 1:2,000
Immunohistochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.19 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
RAB11FIP3
Download CofA:
Lot#  
Print

Rab11 FIB3 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Rab11 Family-Interacting Protein 3 (FIP3)/Arfophilin-1 and FIP4/Arfophilin-2 are dual effectors for Rab11 and the ADP-ribosylation factor Arf6.  Rab11 and Arf6 are involved in membrane delivery from recycling endosomes to the plasma membrane during cytokinesis.  The dual Rab11/Arf6 binding proteins, FIP3 and FIP4, function in the delivery of recycling endosomes to the cleavage furrow and are, together with Rab11, essential for completion of abscission, the terminal step of cytokinesis.  An interaction between Rab11-FIP3 and ASAP1 has also been identified.  ASAP1 is an Arf GTPase-activating protein that has been implicated in the invasive properties of uveal melanoma and mammary carcinoma.
Synonyms
IkB kinase associated protein 1 antibody, IkB kinase subunit gamma antibody, Inhibitor of nuclear factor kappa B kinase subunit gamma antibody, AMCBX1 antibody, FIP 3 antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids spanning the carboxyl terminal one-third of human FIP3.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Although the predicted molecular weight of FIP3 is 82.4 kDa, a band corresponding to FIP3 was observed at 120 kDa in human cell lysates and at 150 kDa in mouse cell lysates.  The identity of a 40 kDa band, observed in western blotting against both HeLa and 293T cell lysates, is unknown (personal communication, P. Randazzo, CCR-NCI, Bethesda, MD).
Purity/Specifity
This affinity purified antibody is directed against human FIP3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross-reactivity with UPLC1/ASAP3 protein from mouse and rat based on approximately 92% protein:protein homology over the region of the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Shiba, T.,  Koga, H., Shin, H.W., Kawasaki, M., Kato, R., Nakayama, K., Wakatsuki, S. (2006) Structural basis for Rab11-dependent membrane recruitment of a family of Rab11-interacting protein 3 (FIP3)/Arfophilin-1. Proc. Natl. Acad. Sci. USA 103(42):15416-15421. Fielding, A.B., Schonteich, E., Matheson, J., Wilson, G., Yu, X., Hickson, G.R., Srivastava, S., Baldwin, S.A., Prekeris, R., Gould, G.W. (2005) Rab11-FIP3 and FIP4 interact with Arf6 and the exocyst to control membrane traffic in cytokinesis. EMBO J. 24(19):3389-3399. Prekeris, R., Davies, J.M., Scheller, R.H. (2001) Identification of a novel Rab11/25 binding domain present in Eferin and Rip proteins. J. Biol. Chem. 276(42):38966-38970. Randazzo, P.A., Andrade, J., Miura, K., Brown, M.T., Long, Y.Q., Stauffer, S., Roller, P., Cooper, J.A. (2000) The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton. Proc. Natl. Acad. Sci. USA 97(8):4011-4016.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF, IP
Reacts With
mouse, human, hamster
Catalog Number
200-501-891

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, mouse, hamster.
Catalog Number
200-501-892

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-994

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry