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Primary Antibodies  >  Phospho Specific Antibodies
 

Rock-2 phospho Y256 Antibody

Rabbit Polyclonal
Code

600-401-998
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's affinity purified anti-ROCK-2 pY256 antibody shows detection of phosphorylated ROCK-2 in transfected 293T cells. Lane 1 shows lysate expressing exogenous ROCK-2 where the insert contains the transversion Y256A.  Lane 2 shows the lysate expressing WT ROCK-2.  A lysate containing a GST null vector also shows no specific staining (data not shown).  Primary antibody was used at a 1:1000 dilution. Personal Communication, A. Papageorge and X. Qian, NCI, Bethesda, MD.
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Synonyms: Rho-associated protein kinase 2 EC=2.7.11.1 Rho-associated, coiled-coil-containing protein kinase 2 p164 ROCK-2 Rho kinase 2

Rock-2 phospho Y256 Antibody Properties

Description
Anti-ROCK-2 (Rho-associated Protein Kinase-2) pY256 (RABBIT) Antibody - 600-401-998
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:20,000
Western Blot : 1:200 - 1:2,000
Immunochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
ROCK2
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Lot#  

Rock-2 phospho Y256 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Progression of tumors to invasive and metastatic forms requires that tumor cells undergo dramatic morphological changes, a process regulated by Rho GTPases.  Increased levels of RhoA or RhoC, as well as the Rho effector proteins ROCK-1 or ROCK-2, were observed in various human carcinomas. In vivo studies have also shown that ROCK inhibition reduced the invasiveness of several tumor cell lines. It is suggested that Rho and ROCK signaling contribute to the morphological changes and metastatic behavior of some tumor cells. Thus, elevated Rho and ROCK expression is associated with tumorigenesis, particularly during the progression to invasive and metastatic phenotypes. Phosphorylation of certain residues in ROCK-2 may be critical for its kinase activity.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 164 kDa in size corresponding to phosphorylated ROCK-2 by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with human ROCK-2 pY256 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide. This antibody is specific for human ROCK-2 protein phosphorylated at Y256.  A BLAST analysis was used to suggest cross-reactivity with ROCK-2 from human, mouse, rat, chicken, dog, bovine, Xenopus and Drosophila based on a 100% homology with the immunizing sequence.  Cross-reactivity with ROCK-2 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Takahashi N, Tuiki H, Saya H and Kaibuchi K (1999) Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24.  Genomics 55 (2): 235-237. Fritz G and Kaina B (2006) Rho GTPases: Promising cellular targets for novel anticancer drugs. Curr. Cancer Drug Targets, 6(1): 1-14. Schwartz M (2004) Rho signalling at a glance. J. Cell Sci. 117(Pt 23): 5457-5458.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Controls;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat. chicken, bovine
Catalog Number
200-301-880

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, chimpanzee, orangutan
Catalog Number
600-401-855

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
Mouse, Rat, Human, Monkey, Sheep, Bovine, Goat, Guinea Pig, Swine, Hamster
Catalog Number
600-401-886

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry