GAPDH Antibody Properties
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody - 600-401-A33
Mouse, Human, Rat, Rabbit, Chicken, Bovine, Swine
ELISA : 1:10,000 - 1:40,000
IF Microscopy : 1:100 - 1:1,000
Western Blot : 1:1,000 - 1:5,000
Immunohistochemistry: 1:100 - 1:1,000
Other Dilution: User Optimized
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
GAPDH loading control antibody is ideal for Western Blotting, ELISA, IHC and IF Microscopy. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control antibody in immunoblots. A loading control antibody is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. Anti-GAPDH antibody is ideal for investigators involved in apoptosis, cancer, DNA damage and repair and neuroscience.
GAPDH Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH).