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Primary Antibodies > Stem Cell Antibodies

Wnt1 Antibody

Rabbit Polyclonal

Code

600-401-A37

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's affinity purified anti-Wnt1 antibody shows detection of endogenous Wnt1 in human-derived MCF7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2). The band at ~41 kDa, indicated by the arrowhead, corresponds to Wnt1. After transfer, the membrane was blocked with 5% BLOTTO. Primary antibody was used at a 1:1,400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with Wnt1 (data not shown).

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Synonyms: INT1 antibody, Murine mammary tumor virus integration site 1 antibody, Oncogene INT1 antibody, Proto oncogene protein Wnt 1 antibody, Wingless type MMTV integration site family member 1 antibody

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Wnt1 Antibody Properties

Description

Anti-Wnt1 (RABBIT) Antibody - 600-401-A37

Host

Rabbit

Target Species

Human

Known Cross Reactivity

mouse, human, rat, bovine, dog, macaque, opossum

Clonality

Polyclonal

Application

ELISA : 1:25,000
Western Blot : 1:1,500 - 1:6,000
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.38 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

WNT1

Wnt1 Antibody Description

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Wnt1 protein.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting.

Purity/Specifity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, and opossum based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

De Ferrari,G.V. and Moon,R.T. (2006) The ups and downs of Wnt signaling in prevalent neurological disorders. Oncogene 25 (57), 7545-7553. Yook,J.I., Li,X.Y., Ota,I., Hu,C., Kim,H.S., Kim,N.H., Cha,S.Y., Ryu,J.K., Choi,Y.J., Kim,J., Fearon,E.R. and Weiss,S.J. (2006) A Wnt-Axin2-GSK3beta cascade regulates Snail1 activity in breast cancer cells. Nat. Cell Biol. 8 (12), 1398-1406. He,J., Sheng,T., Stelter,A.A., Li,C., Zhang,X., Sinha,M., Luxon,B.A. and Xie,J. (2006) Suppressing Wnt signaling by the hedgehog pathway through sFRP-1. J. Biol. Chem. 281 (47), 35598-35602. Tell,S., Yi,H., Jockovich,M.E., Murray,T.G. and Hackam,A.S. (2006) The Wnt signaling pathway has tumor suppressor properties in retinoblastoma. Biochem. Biophys. Res. Commun. 349 (1), 261-269. Sievers,S., Fritzsch,C., Grzegorczyk,M., Kuhnen,C. and Muller,O. (2006) Absolute beta-catenin concentrations in Wnt pathway-stimulated and non-stimulated cells. Biomarkers 11 (3), 270-278.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra