You may also want to consider these related products.
VEGF-165 Human Recombinant Protein - 009-001-B99
VEGF-165 Human Recombinant Protein - 009-001-B99
VEGF-121 Human Recombinant Protein - 009-001-C23
VEGF-121 Human Recombinant Protein - 009-001-C23
Anti-Bovine VEGF-A (RABBIT) Antibody - 200-401-B74
Anti-Bovine VEGF-A (RABBIT) Antibody - 200-401-B74
Anti-Bovine VEGF-A (RABBIT) Antibody - 200-401-B74S
Anti-Bovine VEGF-A (RABBIT) Antibody - 200-401-B74S
Primary Antibodies  >  Cancer Research Antibodies

ABCB5 Antibody

Rabbit Polyclonal


100 µg


Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Immunohistochemistry of ABCB5 antibody
Tissue: human liver
Fixation:  formalin fixed paraffin embedded  
Antigen retrieval:  user optimized
Primary antibody:  ABCB5  1:200
Secondary antibody:  Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT
Localization:  Moderate to strong cytoplasmic and membranous staining was observed in hepatocytes. Occasional nuclear staining was observed in hepatocytes and sinusoidal cells. 
Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain
Western blot using Rockland's affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4° C, overnight. Personal Communication, JP Gillet, CCR-NCI, Bethesda, MD.
Shipping & Handling Fees Apply.

$20.00 to United States
Synonyms: ATP binding cassette sub family B (MDR/TAP) member 5 antibody, ATP binding cassette sub family B member 5 antibody, EST422562 antibody, P glycoprotein ABCB5 antibody

ABCB5 Antibody Properties

Anti-ABCB5 (RABBIT) Antibody - 600-401-A77
Target Species
Known Cross Reactivity
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:20,000
Immunohistochemistry: 1:200
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
1.29 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
Download CofA

ABCB5 Antibody Description

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-ABCB5 Antibody is a ATP-Binding Cassette protein. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5. ABCB5 antibody is ideal for Cancer and Signal Transduction research.
ABCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.
Immunogen Type
Storage Condition
Store Anti-ABCB5 at -20° C prior to opening. Aliquot antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified ABCB5 antibody has been tested for use in ELISA, IHC, and western blotting.  Specific conditions for reactivity should be optimized by the end user.
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human ABCB5 protein.  A BLAST analysis was used to suggest partial cross-reactivity with ABCB5 from monkey (85% homology), rat (68% homology) and mouse (62% homology) sources.  Cross-reactivity with ABCB5 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH. (2005)  ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 65(10):4320-33. Chen KG, Szakacs G, Annereau JP, Rouzaud F, Liang XJ, Valencia JC, Nagineni CN, Hooks JJ, Hearing VJ, Gottesman MM. (2005) Principal expression of two mRNA isoforms (ABCB 5alpha and ABCB 5beta ) of the ATP-binding cassette transporter gene ABCB 5 in melanoma cells and melanocytes.  Pigment Cell Res. 18(2):102-12. Chen KG, Gottesman MM.  (2005) Useful tool to generate unidirectional deletion vectors by utilizing the star activity of BamHI in an NcoI-BamHI-XhoI cassette.  Biotechniques. 38(2):198, 200, 202, 204.
Specific Reference
Log in to submit your product review.

Product Type
Proteins and Peptides;
Reacts With
Catalog Number

Product Type
Proteins and Peptides;
Reacts With
Catalog Number

Product Type
Primary Antibodies;
Reacts With
Catalog Number

Product Type
Primary Antibodies;
Reacts With
Catalog Number

Product Label


Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry