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Primary Antibodies  >  Cancer Research Antibodies
 

Plasminogen Activator Inhibitor 2 Antibody

Rabbit Polyclonal
Code

600-401-B22
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Immunohistochemistry of rabbit anti-Plasminogen Activator 2 antibody.  
Tissue:  heart.  
Fixation:  formalin fixed paraffin embedded.  
Antigen retrieval:  not required.
Primary antibody:  Anti-Plasminogen Activator 2 at 10 µg/mL  for 1 h at RT.
Secondary antibody:  Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.
Staining: Plasminogen Activator II as precipitated red signal with hematoxylin purple nuclear counterstain.
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Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: PAI-2, Placental plasminogen activator inhibitor; Monocyte Arg-serpin; Urokinase inhibitor; SERPINB2 serpin peptidase inhibitor, clade B (ovalbumin), member 2

Plasminogen Activator Inhibitor 2 Antibody Properties

Description
Anti-Plasminogen Activator Inhibitor 2 (RABBIT) Antibody - 600-401-B22
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, monkey
Clonality
Polyclonal
Application
ELISA : 1:100,000 – 150,000
Western Blot : 1:500 – 1:2,000
Immunochemistry: 10µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.2 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SERPINB2
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Lot#  

Plasminogen Activator Inhibitor 2 Antibody Description

Background
Extracellular plasminogen activator inhibitor type-2 (also known as PAI-2, Placental plasminogen activator inhibitor; Monocyte Arg-serpin; Urokinase inhibitor; SERPINB2 serpin peptidase inhibitor, clade B (ovalbumin), member 2) is a coagulation factor and a potent inhibitor of urokinase-type plasminogen activator (u-PA) and also acts as a multifunctional protein. It is present in most cells, especially monocytes and macrophages. PAI-2 exists in two forms, a 60-kDa, secreted, extracellular, glycosylated form and a 43-kDa intracellular form. It is a multifunctional protein that plays a role in cell differentiation, in prevention of programmed cell death, in the regulation of cell proliferation, in the inhibition of microbial proteinases and in the protection against stromal degradation. High levels of the PAI-2 protein are associated with a good prognosis in breast cancer, small cell lung, ovarian cancer, and inhibition of metastasis. PAI-2 also plays a role in inflammation on the surface of the eye. PAI-2 may cooperate with pRb2/p130 in modulating PAI-2 gene expression by chromatin remodeling.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal area of human PAI-2 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, Immunochemistry, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band 42-46.6 kDa in size corresponding to PAI-2 protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity-purified antibody is directed against human PAI-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Reactivity occurs against human PAI-2 protein.   A BLAST analysis was used to suggest cross reactivity with PAI-2 proteins from several primates based on 94% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Macaluso M, Montanari M, Marshall CM, Gambone AJ, Tosi GM, Giordano A, and Massaro-Giordano M. (2006) Cytoplasmic and nuclear interaction between Rb family proteins and PAI-2: a physiological crosstalk in human corneal and conjunctival epithelial cells. Cell Death and Differentiation (2006) 13, 1515–1522. online doi: 10.1038/sj.cdd.4401835.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, monkey
Catalog Number
600-401-B22S

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry