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BrdU Antibody

Rabbit Polyclonal
NCI Collaboration
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  Immunofluorescence microscopy images of paraformaldehyde-fixed, paraffin-embedded pancreas sections stained with antibodies against BrdU (red or pink) and counterstained with DAPI (blue) and imaged with a 40× objective.  DAPI stained nuclei (blue) indicate non-dividing cells, immunostained red and pink nuclei indicate actively dividing pancreatic ß-cells. The antibodies were diluted to 2.7 µg/ml. and incubated with tissue sections overnight at 4 degrees. Donkey anti-rabbit secondary antibody was diluted 1:2500.
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Number:
600-401-C29S
Size:
25 µL
Price:
$95.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

BrdU Antibody Properties

Description
Anti-BrdU (RABBIT) Antibody - 600-401-C29S
Host
Rabbit
Known Cross Reactivity
BrdU
Clonality
Polyclonal
Application
ELISA : 1:2000-1:10,000
IF Microscopy : 1:500
Flow Cytometry : 1:50-1:100
Western Blot : User Optimized
Immunohistochemistry: 1:100-1:500
ImmunoPrecipitation: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
0.72 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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BrdU Antibody Description

Background
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
Synonyms
Bromodeoxyuridine 5-bromo-2'-deoxyuridine, BrdU
Immunogen
Anti-BrdU affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with BromodeoxyUridine-KLH.
Immunogen Type
Other
Storage Condition
Store BrdU Antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This affinity purified antibody has been tested as suitable for immunofluorescence microscopy. Antibody may be suitable for additional immunoassays including western blot, flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incoporated BrdU thymidine analog from replicated cells.
Purity/Specifity
BrdU Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
M. Teta, M. Rankin, S. Long, G. Stein, and J. Kushner. 2007. Growth and Regeneration of Adult ß Cells Does Not Involde Specialized Progenitors. Developmental Cell 12, 817-826, May.
Specific Reference
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Rating
Name
Application
Species
Submitted On
IF Microscopy
Human
6/19/2013 08:56:01 AM
Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Saccharomyces cerevisiae
Catalog Number
600-401-467

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, chimpanzee
Catalog Number
600-401-671


Product Type
Molecular Biology Reagents;
Catalog Number
MB-017

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry