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Primary Antibodies  >  Transcription Factor Antibodies
 

STAT3 phospho Y705 Antibody

Rabbit Polyclonal
Code

600-401-C64
Size

100 µg
Price

$279.00
Availability

Ships next business day.
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Immunohistochemistry of Rabbit anti STAT3 pY705 Antibody.
Tissue:  Human Colon at 40X in colon tissue at pH 9.
Fixation:  formalin fixed paraffin embedded.  
Antigen retrieval:  not required.
Primary antibody:  STAT3 pY705 antibody at 10 µg/mL for 1 h at RT.
Secondary antibody:  Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.
Localization:  STAT3 phospho Y705 is nuclear and cytoplasmic.
Staining:  STAT3 (pTyr705) as precipitated red signal with hematoxylin purple nuclear counterstain.
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Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, STAT3 (phospho Y705), STAT3 (pTry705)

STAT3 phospho Y705 Antibody Properties

Description
Anti-STAT3 pY705 (RABBIT) Antibody - 600-401-C64
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:60,000-1:130,000
Western Blot : 1 µg/mL
Immunochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.9 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
STAT3
Download CofA
Lot#  

STAT3 phospho Y705 Antibody Description

Background
Signal transducer and activator of transcription 3 (Stat3) belongs to a family of cytoplasmic transcription factors that can be activated by phophorylation by its cell surfacereceptor. Stat3 plays a key role in many cellular processes such as cell growth and apoptosis. It also mediates cellular responses to interleukins, KITLG/SCF,EGF, IFN-alpha and other growth factors and may mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Stat3 forms a homodimer or a heterodimer with a related family member (e.g. STAT1). Activation occurs through phosphorylation of tyrosine 705 and serine 727. Phosphorylation of Stat3 at Tyr705 induces Stat3 dimerization and nuclear translocation. Serine phosphorylation is important for stable DNA-binding of Stat3 homodimers and maximal transcriptional activity. Stat3 can have a dual role in cancer, it has been found that Stat3 protein can promote oncogenesis and have a tumor suppressor role depending upon the mutational background of the tumor.
Immunogen
This affinity purified Stat3 antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids surrounding tyrosine 705 of human Stat3.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at-20° C prior to opening. Aliquot contents and freeze at -20° C or below forextended storage. Avoid cycles of freezing and thawing. Centrifuge product ifnot completely clear after standing at room temperature. This product is stablefor several weeks at 4° C as an undiluted liquid. Dilute only prior toimmediate use.
Application Note
STAT3 pY705 antibody has been tested for use in ELISA, IHC, and Western Blot. Expect a band approximately 88.1 kDa in size corresponding to phosphorylated STAT3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-STAT3 antibody is an affinity-purified antibody and is directed against the phosphorylated form of human STAT3 protein at the Y705. Anti-Stat3 antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Anti-Stat3 antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Stat3 protein is minimal by ELISA and western blot. A BLAST analysis was used to suggest crossreactivity with Stat3 antibodies from human, mouse, rat, and pig based on 100%sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
de la Iglesia N, Konopka G, Puram SV, Chan JA, Bachoo RM, You MJ, Levy DE, Depinho RA, Bonni A (2008). ''Identification of a PTEN-regulated STAT3 brain tumor suppressor pathway''. Genes Dev. 22 (4): 449-462. Levy DE, Loomis CA (2007). ''STAT3 signaling and the hyper-IgE syndrome''. N. Engl. J. Med. 357 (16): 1655-1658. Klampfer L (2006). ''Signal transducers and activators of transcription (STATs): Novel targets of chemopreventive and chemotherapeutic drugs''. Curr Cancer Drug Targets 6 (2): 107-121.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse
Catalog Number
100-401-861

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-A45

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse, rat
Catalog Number
200-401-A43

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
600-401-A45

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry