14-10_house_marker_thin
 
You may also want to consider these related products.
Anti-POLK (DNA polymerase kappa) (GOAT) Antibody - 600-101-295
Anti-POLK (DNA polymerase kappa) (GOAT) Antibody - 600-101-295
Anti-Human RAD52 (RABBIT) Antibody - 600-401-679
Anti-Human RAD52 (RABBIT) Antibody - 600-401-679
Anti-MECT1(RABBIT) Antibody - 600-401-936
Anti-MECT1(RABBIT) Antibody - 600-401-936
Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 649 Conjugated - 611-143-002
Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 649 Conjugated - 611-143-002
Primary Antibodies  >  Sample Size Primary Antibodies
 

POLB Antibody

Rabbit Polyclonal
Code

600-401-C65S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western Blot of Rabbit Anti-POLß (DNA polymerase beta) Antibody (600-401-C65S)
Lane 1:  LN428 FLAG POLß (control)
Lane 2:  A172  
Load:  35 µg per lane
Primary antibody: POLß antibody at 1:2000 for overnight at 4°C
Secondary antibody:  IRDye800™ goat anti-rabbit at 1:10,000 for 45 min at RT
Block:  5% BLOTTO  overnight at 4°C
Predicted/Observed size:  ~40 kDa, ~40 kDa 
Other band(s):  unknown band ~75 kDd
Shipping & Handling Fees Apply.

Shipping:
$20.00 to United States
Handling:
$20.00
Synonyms: DNA polymerase beta, POLB, beta-pol

POLB Antibody Properties

Description
Anti-POLB (DNA polymerase beta) (RABBIT) Antibody - 600-401-C65S
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:50,000
Western Blot : 0.5 µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.4 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
POLB
Related Links
Download CofA
Lot#  

POLB Antibody Description

Background
The POLß protein belongs to the DNA polymerase type-X family. It is a DNA polymerase involved in gap-filling DNA synthesis or base excision and repair in a stepwise distributive fashion unlike other DNA polymerases. It is required for DNA maintenance, replication, recombination, and drug resistance. It is normally found as a monomer, in the cytoplasm and it translocates to the nucleus upon DNA damage.
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptidecorresponding to a region near the C-terminus of the POLß protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kDa in size corresponding to POLß by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with overexpressed and endogenous POLß protein. A BLAST analysis was used to suggest reactivity with POLß from human, bovine, and Xenopus laevis based on a 100% homology with the immunizing sequence. Cross-reactivity with POLß from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Tokui,T., Inagaki,M., Nishizawa,K., Yatani,R., Kusagawa,M., Ajiro,K., Nishimoto,Y., Date,T. and Matsukage,A. (1991) Inactivation of DNA polymerase beta by in vitro phosphorylation with protein kinase C. J. Biol. Chem. 266 (17), 10820-10824.
Specific Reference
Sobol, R. W., Foley, J. F., Nyska, A., Davidson, M. G. & Wilson, S. H. (2003) Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice. DNA Repair 2 (Amst). May 13;2(5):609-22. Asagoshi K, Liu Y, Masaoka A, Lan L, Prasad R, Horton JK, Brown AR, Wang XH, Bdour HM, Sobol RW, Taylor JS, Yasui A, Wilson SH. (2010) DNA polymerase beta-dependent long patch base excision repair in living cells. DNA Repair (Amst). Feb 4;9(2):109-19.
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
600-101-295

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
600-401-679

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat, zebrafish, pufferfish, bovine
Catalog Number
600-401-936

Product Type
Secondary Antibodies;
Applications
FLISA, WB, IF
Reacts With
Rabbit
Catalog Number
611-143-002

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry