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Beta Actin Antibody Peroxidase Conjugated

Rabbit Polyclonal
Code

600-403-886
Size

100 µg
Price

$265.00
Availability

Ships next business day.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: HRP, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

Beta Actin Antibody Peroxidase Conjugated Properties

Description
Anti-beta Actin (RABBIT) Antibody Peroxidase Conjugated - 600-403-886
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Mouse, Rat, Human, Monkey, Sheep, Bovine, Goat, Guinea Pig, Swine, Hamster
Clonality
Polyclonal
Application
ELISA : 1:20,000
Western Blot : 1:1,000 - 1:5,000
Immunochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
ACTB
Download CofA
Lot#  

Beta Actin Antibody Peroxidase Conjugated Description

Background
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility.  Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins
Immunogen
Beta Actin Antibody Peroxidase Conjugated antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 359-368 of Human beta Actin.
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Beta Actin Antibody Peroxidase Conjugated antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly.  Beta actin present in neuromuscular junctions also stains.  Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation.  Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
Beta Actin Antibody Peroxidase Conjugated antibody is directed against human beta Actin protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Hu,P., Wu,S. and Hernandez,N. (2004) A role for beta-actin in RNA polymerase III transcription. Genes Dev. 18 (24), 3010-3015. Dahlen,A., et al. (2004) Activation of the GLI oncogene through fusion with the beta-actin gene (ACTB) in a group of distinctive pericytic neoplasms: pericytoma with t(7;12). Am. J. Pathol. 164 (5), 1645-1653. Navarro-Lerida,I., Martinez Moreno,M., Roncal,F., Gavilanes,F., Albar,J.P. and Rodriguez-Crespo,I. (2004) Proteomic identification of brain proteins that interact with dynein light chain LC8. Proteomics 4 (2), 339-346.
Specific Reference
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Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate