House Buffer
 
You may also want to consider these related products.
Bovine COLLAGEN Type IV - 001-001-106
Bovine COLLAGEN Type IV - 001-001-106
Human COLLAGEN Type IV - 009-001-106
Human COLLAGEN Type IV - 009-001-106
Anti-Collagen Type IV (RABBIT) Antibody - 600-401-106-0.1
Anti-Collagen Type IV (RABBIT) Antibody - 600-401-106-0.1
Anti-Collagen Type IV (RABBIT) Antibody - 600-401-106-0.5
Anti-Collagen Type IV (RABBIT) Antibody - 600-401-106-0.5
Primary Antibodies  >  Extracellular Matrix Antibodies
 

Collagen Type IV Antibody Biotin Conjugated

Rabbit Polyclonal
Code

600-406-106
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Immunohistochemistry of Rabbit Anti-Collagen IV Biotin Conjugated Antibody.  
Tissue:  human kidney (Left) with strong red staining observed in glomeruli and liver (Right) with strong staining in sinusoids.  
Fixation:  formalin fixed paraffin embedded.  
Antigen retrieval:  steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes.
Primary antibody:  Collagen IV antibody  at 10 µg/mL  for 1 h at RT.
Secondary antibody:  Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.
Localization: Collagen IV is extracellular.
Staining:  Collagen IV as precipitated red signal with hematoxylin purple nuclear counterstain.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody

Collagen Type IV Antibody Biotin Conjugated Properties

Description
Anti-Collagen Type IV (RABBIT) Antibody Biotin Conjugated - 600-406-106
Host
Rabbit
Target Species
Mammalian
Known Cross Reactivity
human, bovine
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:1,000 - 1:5,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
COL4A4
Download CofA
Lot#  

Collagen Type IV Antibody Biotin Conjugated Description

Background
Anti-Collagen Type IV detects collagen. Type-IV collagen is a type of collagen found primarily in the basal lamina. It is the major structural element of basal membranes. Anti-Collagen Type IV antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
Immunogen
Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.   Expiration date is one (1) year from date of restoration.
Application Note
Anti-Collagen Type IV is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Purity/Specifity
Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.  Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards.  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.   This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens.  Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Log in to submit your product review.

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
001-001-106

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
009-001-106

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, bovine
Catalog Number
600-401-106-0.1

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, bovine
Catalog Number
600-401-106-0.5

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester